Separation of Paraquat, Diguat, and Glyphosate on Obelisc R Column


Paraquat, diquat and glyphosate are three of most widely used herbicides in the world. Paraquat and diquat are very polar and very basic quaternary amines. Glyphosate is an aminophosphonic analog of glycine. It is very polar and acidic at most of the pH of the mobile phase. Since glyphosate and the quats have opposite charges no ion-pairing method can be developed for the mixture of basic and acidic herbicides. All three herbicides were separated on the Obelisc R tri-modal column. Paraquat and diquat are retained by a cation-exchange mechanism, and glyphosate is retained by weak reversed-phase and strong anion-exchange mechanisms. This method can be used for analysis of common herbicides in fruits, vegetables, ground water, drinking water and other matrices. Method is LC/MS compatible and can be used to determine trace levels of herbicides.



Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer Gradient AmFm pH 3.0 - 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound of interest.
Application Analytes:

Application Detection:
UV Detection
ELSD/MS Detection