HPLC Separation of Organics Acids

Primesep D separates organic acids such as fumaric, benzoic, phthalic, naphthoic, and maleic acids by a mixture of anion exchange and reversed phase. Retention times and elution order can be changed by adjusting the percentage of acetonitrile in the mobile. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.


Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV 250 nm


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Fumaric Acid, Benzoic Acid, Phthalic Acid, Maleic Acid, Naphtoic Acid
Application Analytes:
Benzoic Acid
Fumaric Acid
Maleic Acid
Naphthoic Acid
Organic Acids
Phthalic Acid