Separation of Maleic and Succinic Acid on Primesep B2 column

chr_303.gif Organic and inorganic acids can be retained and separated on mixed-mode columns based on weak reversed-phase and weak/medium anion-exchange mechanisms. Amount of ACN, buffer concentration and buffer pH will affect retention time of organic and inorganic acids. Acids can be monitored by low UV, ELSD or LC/MS. Presence of ions is required to facilitate ion-exchange mechanism. Method can be used as a general approach for analysis of acidic hydrophilic and acidic hydrophobic compounds. Carboxylic acids along with inorganic acid can be retained and separated without ion-pairing reagent.


Column Primesep B2, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD, UV 230nm


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Succinic Acid, Maleic Acid

Application Analytes:
Maleic Acid
Organic Acids
Succinic Acid