HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

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Column Newcrom BH, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer H3PO4 – 3%
Flow Rate 0.5 ml/min
Detection UV 275nm


Class of Compounds Acid
Analyzing Compounds Benzenesulfonic, p-Toluenesulfonic Acids


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Benzenesulfonic Acid
Organic Acids
p-Toluenesulfonic Acid (PTSA)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.