| CAS Number | 7440-70-2 |
|---|---|
| Molecular Formula | Ca |
| Molecular Weight | 40.078 |
| InChI Key | FAQLAUHZSGTTLN-UHFFFAOYSA-N |
| Synonyms |
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Applications:
HPLC Analysis of Calcium Ions in Maple Syrup
July 10, 2012
Application Notes: maple syrup primarily consists of sucrose and other sugars, but also has small amounts of amino acids (arginine, threonine, proline), organic acids (malic and fumaric), vitamins (niacin and riboflavin) and traces of minerals (Na, K, Ca, Mn, Mg, Zn ions). One analysis performed on maple syrup is calcium ion determination. A Primesep 100 column was used in cation-exchange mode to isolate calcium ion from other components of the maple syrup. This method can be used for quantification of calcium ion by ELSD/CAD. Limit of detection depends on the type of ELSD/CAD detector used but usually reaches 20 ppm
Application Columns: Primesep 100
Application compounds: calcium ions, Maple Syrup
Detection technique: LC/MS, ELSD/CAD
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Maple Syrup, Calcium |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsOrganic Acids
Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions
July 8, 2011
Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes.
| Column | Primesep N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Potassium, Sodium, Calcium, Glucose |
Application Column
Primesep N
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Glucose
Potassium
Sodium
HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column
October 4, 2005
Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | TFA- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Magnesium, Calcium, Lithium, Barium |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Bromide
Calcium
Lithium
Magnesium
Potassium
Sodium
