HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column

Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.

ColumnPrimesep 100, 4.6×250 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 70/30%
BufferTFA- 0.4%
Flow Rate1.0 ml/min
DetectionELSD 50C
Class of Compounds
Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing CompoundsSodium, Magnesium, Calcium, Lithium, Barium

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
ELSD Detection
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