HPLC ELSD Method for Separation of Metal Ions on Primesep 100 Column

Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.

Column	Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O – 70/30%
Buffer	TFA- 0.4%
Flow Rate	1.0 ml/min
Detection	ELSD 50C

Class of Compounds	Ions, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds	Sodium, Magnesium, Calcium, Lithium, Barium

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Barium
Bromide
Calcium
Lithium
Magnesium
Potassium
Sodium

ELSD Detection

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