Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Nicotinic Acid

CAS Number59-67-6
Molecular FormulaC6H5NO2
Molecular Weight123.111
InChI KeyPVNIIMVLHYAWGP-UHFFFAOYSA-N
LogP0.4
Synonyms
  • niacin
  • Pyridine-3-carboxylic acid
  • 3-pyridinecarboxylic acid
  • 3-Carboxypyridine
  • wampocap
  • vitamin B3
  • Acidum nicotinicum
  • Apelagrin
  • Pellagrin
  • Akotin
  • Daskil
  • Efacin
  • Pelonin
  • Linic
  • nicamin
  • nicobid
  • nicocap
  • nicolar
  • Enduracin
  • Nicodelmine
  • Niconacid
  • Nicotinipca
  • Pellagramin
  • Direktan
  • Niaspan
  • Nicacid
  • Nicangin
  • Peviton
  • Bionic
  • Diacin
  • Nicyl
  • Nyclin
  • Niac
  • Vitaplex N
  • Davitamon PP
  • Nico-Span
  • Tega-Span
  • Nicocidin
  • Nicocrisina
  • Niconazid
  • Nicoside
  • Nicotamin
  • Nicotene
  • Nicovasan
  • Nicovasen
  • Nipellen
  • SK-Niacin
  • Naotin
  • Nicodan
  • Nicodon
  • Niconat
  • Nicosan 3
  • Nicosyl
  • Nicotil
  • Tinic
  • 3-Carboxylpyridine
  • Nicotine acid
  • Niacor

Applications:

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column

March 28, 2024

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column
HPLC Method for Analysis of Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid

Nicotinic acid and nicotinamide are both forms of vitamin B3, also known as niacin. They play crucial roles in the body’s energy metabolism and are essential for maintaining the health of the skin, nervous system, and digestive system.

  1. Nicotinic Acid (Niacin): This is one of the two principal forms of vitamin B3. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.”
  2. Nicotinamide: Also known as niacinamide, this is the other principal form of vitamin B3. Nicotinamide is a precursor to nicotinamide adenine dinucleotide (NAD+), a coenzyme involved in various cellular processes, including energy metabolism. Nicotinamide is often used in skincare products for its anti-inflammatory properties and its ability to improve the appearance of aging skin and reduce hyperpigmentation.

Tranexamic acid, on the other hand, is a synthetic derivative of the amino acid lysine. It is primarily used as an antifibrinolytic agent, meaning it helps to prevent the breakdown of blood clots. Tranexamic acid is commonly used to treat or prevent excessive bleeding, particularly in conditions such as heavy menstrual bleeding, traumatic hemorrhage, and during surgeries where significant blood loss is expected. Additionally, it has been investigated for its potential role in treating melasma, a common skin condition characterized by hyperpigmentation.

Each of these compounds has distinct biochemical properties and medical applications, but they all play important roles in human health and medicine.

Nicotinic Acid, Nicotinamide, Tranexamic Acid can be retained and separated using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN 40%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 210nm

Class of Compounds
Acids
Analyzing CompoundsNicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Tranexamic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Simultaneous Determination of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate on Primesep 100 Column

August 29, 2022

Separation type: Liquid Chromatography Mixed-mode



High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient that plants and animals synthesize from tryptophan. It is also used as a dietary supplement to treat pellagra. A derivative of Nicotinic acid, Nictotinamide, is a key component of the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP+). Pyridine Dicarboxylic acid (PDA), or Dipicolinic acid (DPA), is a key factor in bacterial endospore heat resistance. It also plays an important role in preparing transition metal complexes for ion chromatography. Ethyl Methyl Pyridine is a common precursor to Nicotinic acid. Ethyl nicotinate, or nicotine acid ethyl ester, is a popular topical treatment for ligament, joint, and muscle pain. These 5 organic compounds can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an analytical method with a simple gradient mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – Gradient
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV 250 nm

 

Description

Class of Compounds Drug, Acid
Analyzing Compounds Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
2,5-Pyridinedicarboxylic acid
5-Ethyl-2-methylpyridine
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridinedicarboxylic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Maleic acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid on BIST™ A+ Column

July 8, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Organic Acids on BIST™ A+ Column
HPLC Method for Analysis of Maleic acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid on BIST™ A+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Maleic  acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid

The maleate ion from Maleic acid is a popular ingredient as the maleate salt in several different drugs, including Methergine, Pyrilamine, and Carfenazine, among others. Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient for the human body and is sometimes taken as a treatment for high cholesterol. Aconitic acid is a key intermediary in the citric acid cycle, and is also used a flavoring agent and in the production of rubbers and plastics. Fumaric acid is a popular preservative and food additive with a fruit-like taste. Using SIELC’s newly introduced BIST™ method, a mixture of these organic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these organic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.

https://bist.lc/shop/columns/bist/bist-a-bist/bist-a-column-4-6x150mm-5um-100a-2/

Condition

ColumnBIST™ A+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN Gradient
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) phosphate  – 5 mM pH 4.0
Flow Rate1.0 ml/min
DetectionUV 250 nm

Description

Class of CompoundsAcids, Organic acid
Analyzing CompoundsMaleic  acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid

Application Column

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
Ascorbic Acid
Fumaric Acid
Maleic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Oxalic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Maleic  Acid, Nicotinic Acid, Aconitic Acid and Fumaric Acid on BIST™ A+ Column

July 7, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Organic Acids on BIST™ A+ Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of  Maleic  Acid, Nicotinic Acid, Aconitic Acid, Fumaric Acid

The maleate ion from Maleic acid is a popular ingredient as the maleate salt in several different drugs, including Methergine, Pyrilamine, and Carfenazine, among others. Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient for the human body and is sometimes taken as a treatment for high cholesterol. Aconitic acid is a key intermediary in the citric acid cycle, and is also used a flavoring agent and in the production of rubbers and plastics. Fumaric acid is a popular preservative and food additive with a fruit-like taste. Using SIELC’s newly introduced BIST™ method, a mixture of these organic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes.

Condition

ColumnBIST™ A+, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 70%
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) Phosphate  – 10 mM pH 4.0
Flow Rate1.0 ml/min
DetectionUV 200 nm

Description

Class of Compounds
Acid, Dicarboxylic acid, Tricarboxylic acid, Pyridinecarboxylic acid
Analyzing CompoundsMaleic  Acid, Nicotinic Acid, Aconitic Acid, Fumaric Acid

Application Column

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Aconitic acid
Fumaric Acid
Maleic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
trans-Aconitic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation Of Mixture of Pyridylacetic Acids And Their Derivatives

May 23, 2018

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
2-Pyridylacetic acid hydrochloride
3-Acetylpyridine
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridyl-3-thioacetomorpholide
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column

July 6, 2015


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Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

Condition

Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
 Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide, Vitamin B3

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Niacin
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Pyridinecarboxylic Acids

March 27, 2011


Pyridinecarboxylic acids exist as three isomers with different position of carboxylic acid relative to nitrogen in pyridine. Three isomers of pyridinecarboxylic acid (picolinic or 2-pyridinecarvoxylic acid, niacin or 3-pyridinecarboxylic acid, isonicotinic or 4-pyridinecarboxylic acid), along with pyridinedicarboxylic acid, are separated on a Primesep 100 column. Pyridinecarboxylic acids have a similar empirical formula, and are very similar in terms of hydrophobicity and ionic properties. Small differences in these properties are enough to achieve good separation on cation-exchange mixed-mode HPLC column like Primesep 100. Retention time for all compounds is controlled by the amount of acetonitrile and amount of ions in the mobile phase. Ions in the mobile phase can be created by organic and inorganic acids and corresponding salt buffers. Various detection techniques can be used for monitoring pyridinecarboxylic acids. Other ionizable isomers can be successfully separated on mixed-mode columns.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Organic Acids
Picolinic Acid
Pyridinedicarboxylic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities

August 22, 2008

Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.

Condition

Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Ascorbic acid, Niacinamide, Pantothenic acid, Caffeine, Riboflavin, Barbituric Acid,  3- Aminopyrine

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:

3-Aminopyridine
4-Aminopyridine/Fampridine
Ascorbic Acid
Barbituric Acid
Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Carboxylic Acids

January 13, 2005


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Condition 1

Column Newcrom  B, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O- 30/70%
Buffer H3PO4 – 0.5%
Flow Rate 0.5 ml/min
Detection UV, 275 nm

 


HPLC separation of carboxylic acids (nicotinic acid, benzoic acid and acetylbenzoic acid) on Primesep B2 column using an isocratic method by reverse-phase and anion-exchange mechanisms. The elution of carboxylic acids using the same isocratic method and mobile phase can be reversed by using a reverse-phase cation-exchange mechanisms of the Primesep 100 column. The mobile phase is water, acetonitrile (MeCN, ACN) and formic acid with a UV detector.

Condition 2

Column Primesep B2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Acid, Hydrophilic
Analyzing Compounds Nicotinic acid, Benzoic acid, Acetylbenzoic acid,

1.4-acetylbenzoic Acid

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Acetylbenzoic Acid
Benzoic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.