Cyanocobalamin

 

CAS Number68-19-9
Molecular FormulaC63H88CoN14O14P
Molecular Weight1355.4
InChI KeyFDJOLVPMNUYSCM-WZHZPDAFSA-L
Synonyms
  • vitamin B12
  • cyanocobalamin
  • 68-19-9
  • Vitamin B12 (charged)
  • Cyanocob(III)alamin
  • Vitamin B12 complex
  • DSSTox_CID_24346
  • DSSTox_RID_80159
  • DSSTox_GSID_44346
  • C63H88CoN14O14P
  • CAS-68-19-9
  • NCGC00016302-01
  • Prestwick_564
  • Vitamin B12 NOS
  • DTXSID7044346
  • HMS1569G08
  • HMS2096G08
  • HMS3713G08
  • Tox21_110358
  • Tox21_113664
  • AKOS015894306
  • CCG-220433
  • DB00115
  • NCGC00249888-01
  • H486
  • V0344
  • Q55167869
  • Cobinamide,dihydrogenphosphate(ester),inner salt,3'-ester with(5,6-Dimethyl-1-a-d-ribofuranosyl-1H-benzimidazole-kn3),ion(1+)(9ci)

Applications:

HPLC Determination of Cyanocobalamin (Vitamin B-12) on PEI Column

December 21, 2020


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Vitamin B12 is a water-soluble vitamin that is naturally present in some foods and available as a dietary supplement and as prescription medication. Vitamin B12 exists in several forms and contains the mineral cobalt, so compounds with vitamin B12 activity are collectively called “cobalamins”. It plays an essential role in the production of the red blood cells and DNA, as well as in the proper functioning of the nervous system.
Vitamin B12 can be retained and UV detected at 360nm.Using the PEI column and a mobile phase gradient of organic modifier and phosphoric acid (H3PO4) as buffer.

Condition
Column PEI, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer H3PO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV 360 nm

 

Description
Class of Compounds
 Vitamin, Drug
Analyzing Compounds Cyanocobalamin (Vitamin B-12)

 

Application Column

PEI

SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

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Application Analytes:
Cyanocobalamin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.