Pantothenic Acid

Panthothenic Acid

CAS Number79-83-4
Molecular FormulaC9H17NO5
Molecular Weight219.237
  • Vitamin B5
  • pantothenic acid
  • D-pantothenic acid
  • 79-83-4
  • pantothenate
  • Chick antidermatitis factor
  • (+)-Pantothenic acid
  • (R)-pantothenate
  • Kyselina pantothenova [Czech]
  • calcium pantothenate
  • HSDB 1020
  • Pantothenic Acid [BAN]
  • D(+)-N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-beta-alanine
  • (D)-(+)-Pantothenic acid
  • BRN 1727064
  • (R)-pantothenic acid
  • UNII-19F5HK2737
  • Zinc pantothenate
  • beta-Alanine, N-(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)-, (R)-
  • D-(+)-pantothenic acid
  • EINECS 201-229-0
  • (D,+)-N(alpha-gamma-Dihydroxy-beta,beta-dimethylbutyryl)-beta-alanine
  • N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-beta-alanine
  • CHEBI:46905
  • 19F5HK2737
  • (R)-3-(2,4-Dihydroxy-3,3-dimethylbutanamido)propanoic acid
  • AK116516
  • (R)-N-(2,4-Dihydroxy-3,3-dimethyl-1-oxobutyl)-beta-alanine
  • Pantothenic acid (BAN)
  • Pantothenic acid, D-
  • (+)-Pantothenate
  • 3-[[(2R)-2,4-dihydroxy-3,3-dimethylbutanoyl]amino]propanoic acid
  • Pantothenate, Zinc
  • PAU
  • B5, Vitamin
  • Vitamin B 5
  • Kyselina pantothenova
  • B 5, Vitamin
  • Pantothenate, Calcium
  • d-(+)-pantothenate
  • Pantothen Pharmaselect
  • pantothenate;vitamin B5


HPLC Analysis of Active Drug in a Formulation

October 4, 2010

HPLC method for separation of active ingredients of drug/supplemental composition was developed on an Obelisc R trimodal HPLC column. Compounds are retained by combination of reversed-phase, cation-exchange and anion-exchange mechanisms. Compounds are well separated, and method can be used for quantitation of pyridoxine, ascorbic acid, niacinamide, pantothenic acid, caffeine and riboflavin in a mixture or as separate compounds in various complex mixtures. Various detection techniques can be applied for quantitation (ELSD, UV, LC/MS, Corona). This HPLC method can be adopted as general approach for analysis of active drug components in various formulations.


Column Obelisc R , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O  -5/95%
Buffer NaHPO4 pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection UV, 210 nm



Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Ascorbic acid, Niacinamide, Pantothenic acid, Caffeine, Riboflavin

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:

Ascorbic Acid
Pantothenic Acid
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.