Vitamin B1 (Thiamine)

Vitamin B1 (Thiamine) structural formula

CAS Number59-43-8
Molecular FormulaC12H17ClN4OS
Molecular Weight300.810
  • Thiamine
  • 3-[(4-Amino-2-methylpyrimidin-5-yl)methyl]-5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium chloride
  • Thiazolium, 3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methyl-, chloride (1:1)
  • 59-43-8
  • Vitamin B1
  • Thiazolium, 3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methyl- chloride
  • 3-[(4-Amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methylthiazolium chloride
  • Aneurine
  • Apatate Drape
  • Bethiamin
  • Oryzanin
  • Thiacoat
  • Thiamin
  • Thiamine monochloride
  • Thiazolium, 3-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-5-(2-hydroxyethyl)-4-methyl- chloride (1:1)
  • tiamina
  • Vitaneurin
  • 3-((4-Amino-2-methyl-5-pyrimidinyl)methyl)-5-(2-hydrox yethyl)-4-methylthiazolium chloride
  • 3-((4-Amino-2-methyl-5-pyrimidinyl)methyl)-5-(2-hydroxyethyl)-4-methylthiazolium chloride
  • EINECS 200-425-3
  • Oryzanine
  • Thiaminum
  • UNII-X66NSO3N35
  • thiamine(1+) chloride
  • 100660-17-1
  • 115461-66-0
  • 55463-15-5
  • 57777-32-9


HILIC Separation of Vitamins Group B

Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.


Column Obelisc N , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAC pH 4.5
Flow Rate 1.0 ml/min
Detection UV, 270 nm, ELSD


Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine,  Niacinamide, Pantothenic acid, Riboflavin

Application Analytes:
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection
ELSD/MS Detection

HPLC Separation of Vitamin B

Primesep C separates B vitamins with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. Vitamin B1 (thiamine), vitamin B2 (riboflavin), and vitamin B6 (pyridoxine) are separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 280 nm.


Column Primesep C , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O  -10/90%
Buffer NEt3H3PO4 Ph 3.0 - 20 Mm
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Thiamine, Riboflavin

Application Analytes:

Riboflavin (Vitamin B2)
Thiamine (Vitamin B1)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection