Nicotinamide

High Performance Liquid Chromatography (HPLC) Method for Analysis of Water-Soluble Vitamins (B1, B2, B3, B6) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate

Water-Soluble Vitamins (B1, B2, B3, B6) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 300 nm.

You can find detailed UV spectra of Vitamin B1 (Thiamine), Vitamin B2 (Riboflavin), Nicotinamide, Vitamin B6 (Pyridoxine), Pyridoxamine, Pyridoxal Phosphate and information about its various lambda maxima by visiting the following links: Vitamins (B1, B2, B3, B6)

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide, Vitamin B6 (Pyridoxine)

Nicotinamide is a specific form of vitamin B3, an essential nutrient that is crucial for various bodily functions. Along with niacin (nicotinic acid), nicotinamide is one of the primary forms of vitamin B3 that the body uses. Both of these forms are converted into coenzymes NAD+ and NADP+, which play vital roles in energy production, DNA repair, and cellular communication. This conversion is essential for maintaining the body’s metabolic processes, highlighting the importance of nicotinamide as a form of vitamin B3.

Pyridoxine is a form of vitamin B6, which is essential for numerous biochemical processes in the body. Vitamin B6 exists in three natural forms—pyridoxine, pyridoxal, and pyridoxamine—all of which are converted into the active coenzyme pyridoxal 5′-phosphate (PLP). This active form is critical for amino acid metabolism, neurotransmitter synthesis, and hemoglobin production. As a form of vitamin B6, pyridoxine is key to maintaining proper neurological and immune function, ensuring that the body can perform a wide range of essential functions.

Nicotinamide, Vitamin B6 (Pyridoxine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 270 nm.

You can find detailed UV spectra of Nicotinamide, Vitamin B6 (Pyridoxine) and information about its various lambda maxima by visiting the following links: UV-Vis Spectrum of Nicotinamide, UV-Vis Spectrum of Pyridoxine.

HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Tranexamic acid

Nicotinic acid and nicotinamide are both forms of vitamin B3, also known as niacin. They play crucial roles in the body’s energy metabolism and are essential for maintaining the health of the skin, nervous system, and digestive system.

1. Nicotinic Acid (Niacin): This is one of the two principal forms of vitamin B3. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.”
2. Nicotinamide: Also known as niacinamide, this is the other principal form of vitamin B3. Nicotinamide is a precursor to nicotinamide adenine dinucleotide (NAD+), a coenzyme involved in various cellular processes, including energy metabolism. Nicotinamide is often used in skincare products for its anti-inflammatory properties and its ability to improve the appearance of aging skin and reduce hyperpigmentation.

Tranexamic acid, on the other hand, is a synthetic derivative of the amino acid lysine. It is primarily used as an antifibrinolytic agent, meaning it helps to prevent the breakdown of blood clots. Tranexamic acid is commonly used to treat or prevent excessive bleeding, particularly in conditions such as heavy menstrual bleeding, traumatic hemorrhage, and during surgeries where significant blood loss is expected. Additionally, it has been investigated for its potential role in treating melasma, a common skin condition characterized by hyperpigmentation.

Each of these compounds has distinct biochemical properties and medical applications, but they all play important roles in human health and medicine.

Nicotinic Acid, Nicotinamide, Tranexamic Acid can be retained and separated using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient that plants and animals synthesize from tryptophan. It is also used as a dietary supplement to treat pellagra. A derivative of Nicotinic acid, Nictotinamide, is a key component of the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP+). Pyridine Dicarboxylic acid (PDA), or Dipicolinic acid (DPA), is a key factor in bacterial endospore heat resistance. It also plays an important role in preparing transition metal complexes for ion chromatography. Ethyl Methyl Pyridine is a common precursor to Nicotinic acid. Ethyl nicotinate, or nicotine acid ethyl ester, is a popular topical treatment for ligament, joint, and muscle pain. These 5 organic compounds can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an analytical method with a simple gradient mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

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Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.

A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH