HPLC Method for Analysis of Nicotinamide on Primesep 100 Column on Alltesta™

HPLC Method for Nicotinamide on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinamide

Nicotinamide, also known as niacinaminde, is a form of vitamin B3 with the chemical formula C6H6N2O2. It is preferred treatment for pellagra and is a common treatment for acne due to it’s anti-inflammatory properties. If consumed daily, it is shown to decrease the risk of skin cancers other than melanoma.

Nicotinamide can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsVitamin
Analyzing CompoundsNicotinamide

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Nicotinamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.