Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column View on Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH  was used to separate nicotinamide and nicotinic acid.  Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.


Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H3PO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds  Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Nicotinic Acid, Nicotinamide, Vitamin B3
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Application Analytes:
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection