2,5-Pyridinedicarboxylic acid

2,5-Pyridinedicarboxylic acid structural formula

CAS Number100-26-5
Molecular FormulaC7H5NO4
Molecular Weight167.120
InChI KeyLVPMIMZXDYBCDF-UHFFFAOYSA-N
LogP0.307
Synonyms
  • 2,5-Pyridinedicarboxylic acid
  • Pyridine-2,5-dicarboxylic acid
  • 100-26-5
  • EINECS 202-834-2
  • NSC 177
  • Pyridine 2,5-dicarboxylate
  • UNII-4K1KGR926X
  • Isocinchomeronic acid
  • pyridinedicarboxylic acid-(2,5)
  • 1355602-04-8

Applications:

HPLC Method for Simultaneous Determination of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate on Primesep 100 Column

August 29, 2022

Separation type: Liquid Chromatography Mixed-mode



High Performance Liquid Chromatography (HPLC) Method for Analysis of Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient that plants and animals synthesize from tryptophan. It is also used as a dietary supplement to treat pellagra. A derivative of Nicotinic acid, Nictotinamide, is a key component of the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP+). Pyridine Dicarboxylic acid (PDA), or Dipicolinic acid (DPA), is a key factor in bacterial endospore heat resistance. It also plays an important role in preparing transition metal complexes for ion chromatography. Ethyl Methyl Pyridine is a common precursor to Nicotinic acid. Ethyl nicotinate, or nicotine acid ethyl ester, is a popular topical treatment for ligament, joint, and muscle pain. These 5 organic compounds can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an analytical method with a simple gradient mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – Gradient
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV 250 nm

 

Description

Class of Compounds Drug, Acid
Analyzing Compounds Nicotinic Acid, Nicotinamide, Pyridine Dicarboxylic Acid, Ethyl Methyl Pyridine and Ethyl Nicotinate

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2,5-Pyridinedicarboxylic acid
5-Ethyl-2-methylpyridine
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridinedicarboxylic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 2,5-Pyridinedicarboxylic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of 2,5-Pyridinedicarboxylic acid on Newcrom C18 HPLC column

2,5-Pyridinedicarboxylic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
2,5-Pyridinedicarboxylic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.