Nicotinamide Adenine Dinucleotide (NAD)

Nicotinamide Adenine Dinucleotide

 

CAS Number53-84-9
Molecular FormulaC21H27N7O14P2
Molecular Weight663.430
InChI KeyBAWFJGJZGIEFAR-NNYOXOHSSA-N
LogP-4.17
Synonyms
  • Nadide
  • 53-84-9
  • 5-26-16-00399
  • Adenosine 5'-(trihydrogen diphosphate), P'-5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium, inner salt
  • Adenine-nicotinamide dinucleotide
  • Adenosine 5'-(trihydrogen diphosphate), 5'-5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium hydroxide, inner salt
  • Adenosine 5'-(trihydrogen diphosphate), P'-5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium hydroxide, inner salt
  • Codehydrase I
  • Codehydrogenase I
  • Coenzyme I
  • Cozymase I
  • Diphosphopyridine nucleotide
  • Enzopride
  • Nicotinamide adenine dinucleotide
  • Nicotinamide-adenine dinucleotide
  • NSC 20272
  • Oxidized diphosphopyridine nucleotide
  • Pyridinium, 3-carbamoyl-1-β-D-ribofuranosyl-, hydroxide, 5'-5'-ester with adenosine 5'-(trihydrogen pyrophosphate), inner salt
  • β-Diphosphopyridine nucleotide
  • β-NAD+
  • β-Nicotinamide adenine dinucleotide
  • β-Nicotineamide adenine dinucleotide
  • BRN 3584133
  • EINECS 200-184-4
  • Nicotinamide dinucleotide
  • Nicotineamide adenine dinucleotide
  • Pyridine, nucleotide diphosphate
  • beta-Diphosphopyridine nucleotide
  • beta-NAD
  • beta-NAD+
  • beta-Nicotinamide adenine dinucleotide
  • Nadidum
  • UNII-0U46U6E8UK
  • 1083039-67-1
  • 1354693-98-3
  • 159929-29-0
  • 30429-30-2

Applications:

HPLC Separation of NAD and NADH on PEI Column

July 22, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of SNAD and NADH

Nicotinamide adenine dinucleotide (NAD), is a coenzyme found in every single living cell. NAD can exist in two forms: NAD+ and NADH. The conversion of NAD from its oxidized form (NAD+) to its reduced form (NADH), and back, provides the cell with a mechanism for accepting and donating electrons. NAD and NADH can be retained, separated and UV detected at 260 nm using the PEI column with a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV, ELSD, CAD or LC/MS.

Condition
Column PEI, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Ammonium Acetate pH 6.8 – 60 mM
Flow Rate 2.0 ml/min
Detection UV 260 nm

Description
Class of Compounds
  Drug
Analyzing Compounds NAD, NADH

Application Column

PEI

SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

Select options
Application Analytes:
Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Dinucleotide (reduced) (NADH)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Nicotinamide and Related Substances

May 11, 2015

A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
1-Methylnicotinamide
Adenosine Monophosphate
Nicotinamide
Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Mononucleotide
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.