HPLC Separation of NAD and NADH on PEI Column

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of SNAD and NADH

Nicotinamide adenine dinucleotide (NAD), is a coenzyme found in every single living cell. NAD can exist in two forms: NAD+ and NADH. The conversion of NAD from its oxidized form (NAD+) to its reduced form (NADH), and back, provides the cell with a mechanism for accepting and donating electrons. NAD and NADH can be retained, separated and UV detected at 260 nm using the PEI column with a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV, ELSD, CAD or LC/MS.

Condition
Column PEI, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Ammonium Acetate pH 6.8 – 60 mM
Flow Rate 2.0 ml/min
Detection UV 260 nm

Description
Class of Compounds
  Drug
Analyzing Compounds NAD, NADH

Application Column

PEI

SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

Select options
Application Analytes:
Nicotinamide Adenine Dinucleotide (NAD)
Nicotinamide Adenine Dinucleotide (reduced) (NADH)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.