CAS Number | 58-85-5 |
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Molecular Formula | C10H16N2O3S |
Molecular Weight | 244.310 |
InChI Key | YBJHBAHKTGYVGT-ZKWXMUAHSA-N |
LogP | 0.558 |
Synonyms |
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Applications:
HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni Column
September 17, 2025
HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for separation of Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin
Riboflavin (B2), Nicotinic Acid (B3), Nicotinamide (B3), Pyridoxine (B6), Folic Acid (B9), Cyanocobalamin (B12), Thiamine Pyrophosphate (coenzyme of B1), Thiamine (B1), Pantothenic Acid (B5), Biotin (B7) are water soluble vitamins with a key function of energy metabolism. These coenzymes are responsible for converting food into usable energy.
Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.
Column | Chromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
Mobile Phase | Gradient of MeCN |
Buffer | Gradient of Phosphoric Acid |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm, 200 nm |
Class of Compounds | Vitamins |
Analyzing Compounds | Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin |
Application Column
Chromni™
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Cyanocobalamin
Folic Acid
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pantothenic Acid
Thiamine diphosphate (Thiamine pyrophosphate)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
HPLC Determination of Biotin (Vitamin B7) on Newcrom BH Column
May 17, 2021
Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Biotin (Vitamin B7)
Biotin (Vitamin B7) is a water-soluble vitamin that helps your body breakdown key nutrients in order for it to absorb and utilize them. A lack of biotin can lead to hair loss, rash, and brittle nails. A healthy diet including eggs, milk, and bananas can supply your body with a healthy dose of biotin.
Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile with an ammonium formate (AmFm) buffer, Vitamin B7 can be retained and measured. Vitamin B7 can be detected using charged aerosol detection (CAD) with high resolution.
Column | Newcrom BH, 4.6×150 mm, 3 µm, 100A |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | AmFm – pH 3.0 – 10 mm |
Flow Rate | 1.0 ml/min |
Detection | CAD |
Class of Compounds |
Vitamin |
Analyzing Compounds | Biotin, Vitamin B7 |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsSeparation of Biotin on Newcrom R1 HPLC column
February 19, 2018
Biotin, also known as vitamin B7, is a micronutrient produced by the body that helps break down carbohydrates, fatty acids. It also helps form sugar from non-carbohydrate substances.
Biotin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible, applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options