HPLC Determination of Biotin (Vitamin B7) on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Biotin (Vitamin B7)







Biotin (Vitamin B7) is a water-soluble vitamin that helps your body breakdown key nutrients in order for it to absorb and utilize them. A lack of biotin can lead to hair loss, rash, and brittle nails. A healthy diet including eggs, milk, and bananas can supply your body with a healthy dose of biotin.
Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile with an ammonium formate (AmFm) buffer, Vitamin B7 can be retained and measured. Vitamin B7 can be detected using charged aerosol detection (CAD) with high resolution.



Condition
Column Newcrom BH, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm – pH 3.0 – 10 mm
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
 Vitamin
Analyzing Compounds Biotin, Vitamin B7

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Biotin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.