Biotin

Biotin structural formula

CAS Number58-85-5
Molecular FormulaC10H16N2O3S
Molecular Weight244.310
InChI KeyYBJHBAHKTGYVGT-ZKWXMUAHSA-N
LogP0.558
Synonyms
  • Biotin
  • 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid
  • 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, (3aS,4S,6aR)-
  • 58-85-5
  • D-(+)-Biotin
  • D-Biotin
  • 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, (3aS,4S,6aR)-
  • (+)-Biotin
  • (+)-cis-Hexahydro-2-oxo-1H-thieno[3,4]imidazole-4-valeric acid
  • [3aS-(3a?,4?,6a?)]-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanoic acid
  • 1H-THIENO(3,4-D)IMIDAZOLE-4-PENTANOIC ACID, HEXAHYDRO-2-OXO-, (3AS-(3A,alpha.,4?,6A?)
  • 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, [3aS-(3a?,4?,6a?)]-
  • 5-(3AS,4S,5AR)-HEXAHYDRO-2-OXO-1H-THINO(3,4)- DIIMIDAZOL-4-YL) (3aS,4S,6aR)-
  • Biodermatin
  • Bioepiderm
  • Bios II
  • BIOTIN, D-
  • biotina
  • Biotine
  • cis-(+)-Tetrahydro-2-oxothieno[3,4]imidazoline-4-valeric acid
  • Coenzyme R
  • D(+)-Biotin
  • Factor S
  • Meribin
  • NSC 63865
  • Rovimix H 2
  • Vitamin H
  • EINECS 200-399-3
  • Hexahydro-2-oxo-1H-thieno(3,4-d)imidazole-4-pentanoic acid, (3aS-(3aalpha,4beta,6aalpha))-
  • Injacom H
  • 2'-Keto-3,4-imidazolido-2-tetrahydrothiophene-N-valeric acid
  • cis-Tetrahydro-2-oxothieno(3,4-d)imidazoline-4-valeric acid
  • 1H-Thieno(3,4-d)imidazole-4-pentanoic acid, hexahydro-2-oxo-, (3aS-(3aalpha,4beta,6aalpha))-
  • Vitamin Bw
  • beta-Biotin
  • Medebiotin
  • Biotinum
  • UNII-6SO6U10H04
  • (+)-cis-Hexahydro-2-oxo-1H-thieno[3,4]imidazole-4-valerate
  • (3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-D]imidazole-4-valerate
  • (3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-valeric acid
  • -(+)-biotin
  • 1swk
  • 1swn
  • 1swr
  • 5-(2-Oxohexahydro-1H-thieno[3,4-D]imidazol-4-yl)pentanoate
  • 5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid
  • 5-[(1R,5S,6S)-3-oxo-7-thia-2,4-diazabicyclo[3.3.0]oct-6-yl]pentanoic acid
  • 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazolidin-4-yl]pentanoic acid
  • Bioepiderm
  • Bios h
  • D-Biotin factor S
  • Factor S (vitamin)
  • Hexahydro-2-oxo-1H-thieno(3,4-D)imidazole-4-pentanoate
  • Hexahydro-2-oxo-1H-thieno(3,4-D)imidazole-4-pentanoic acid
  • Hexahydro-2-oxo-[3aS-(3aa,4b,6aa)]-1H-Thieno[3,4-D]imidazole-4-pentanoate
  • Hexahydro-2-oxo-[3aS-(3aa,4b,6aa)]-1H-Thieno[3,4-D]imidazole-4-pentanoic acid
  • Hexahydro-2-oxo-[3as-(3alpha,4beta,6alpha)]-1H-Thieno[3,4-D]imidazole-4-pentanoate
  • Hexahydro-2-oxo-[3as-(3alpha,4beta,6alpha)]-1H-Thieno[3,4-D]imidazole-4-pentanoic acid
  • Lutavit H2
  • Vitamin-h
  • cis-(+)-Tetrahydro-2-oxothieno[3,4]imidazoline-4-valerate
  • cis-Hexahydro-2-oxo-1H-thieno(3,4)imidazole-4-valeric acid
  • cis-Tetrahydro-2-oxothieno(3,4-d)imidazoline-4-valeric acid
  • delta-(+)-Biotin
  • delta-Biotin
  • delta-Biotin factor S
  • vitamin B7
  • 15720-24-8
  • 22879-79-4
  • 3672-05-7
  • 58073-87-3

Applications:

HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni Column

September 17, 2025

HPLC Method for Separation of 10 Water-Soluble Vitamins on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for separation of Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin

Riboflavin (B2), Nicotinic Acid (B3), Nicotinamide (B3), Pyridoxine (B6), Folic Acid (B9), Cyanocobalamin (B12), Thiamine Pyrophosphate (coenzyme of B1), Thiamine (B1), Pantothenic Acid (B5), Biotin (B7) are water soluble vitamins with a key function of energy metabolism. These coenzymes are responsible for converting food into usable energy.

Vitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN). Detection is performed using UV.

ColumnChromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseGradient of MeCN
BufferGradient of Phosphoric Acid
Flow Rate1.0 ml/min
DetectionUV 275 nm, 200 nm

Class of CompoundsVitamins
Analyzing CompoundsVitamin B2 (Riboflavin), Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), Nicotinamide, Vitamin B6 (Pyridoxine), Folic Acid, Cyanocobalamin, Thiamine diphosphate (Thiamine pyrophosphate), Vitamin B1 (Thiamine), Pantothenic Acid, Biotin

Application Column

Chromni™

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Biotin
Cyanocobalamin
Folic Acid
Nicotinamide
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pantothenic Acid
Thiamine diphosphate (Thiamine pyrophosphate)
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Biotin (Vitamin B7) on Newcrom BH Column

May 17, 2021

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Biotin (Vitamin B7)







Biotin (Vitamin B7) is a water-soluble vitamin that helps your body breakdown key nutrients in order for it to absorb and utilize them. A lack of biotin can lead to hair loss, rash, and brittle nails. A healthy diet including eggs, milk, and bananas can supply your body with a healthy dose of biotin.
Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile with an ammonium formate (AmFm) buffer, Vitamin B7 can be retained and measured. Vitamin B7 can be detected using charged aerosol detection (CAD) with high resolution.



Condition
Column Newcrom BH, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm – pH 3.0 – 10 mm
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
 Vitamin
Analyzing Compounds Biotin, Vitamin B7

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Biotin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Biotin on Newcrom R1 HPLC column

February 19, 2018
Separation of Biotin on Newcrom C18 HPLC column

Biotin, also known as vitamin B7, is a micronutrient produced by the body that helps break down carbohydrates, fatty acids. It also helps form sugar from non-carbohydrate substances.
Biotin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible, applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Biotin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.