HPLC Method for Separation of Nicotinic Acid, Nicotinamide, Tranexamic Acid on Primesep 100 Column by SIELC Technologies

Nicotinic acid and nicotinamide are both forms of vitamin B3, also known as niacin. They play crucial roles in the body’s energy metabolism and are essential for maintaining the health of the skin, nervous system, and digestive system.

Nicotinic acid, also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is an essential nutrient that is crucial for energy production. Nicotinic acid is commonly used as a dietary supplement to treat vitamin B3 deficiency, and it’s also prescribed in higher doses to help lower cholesterol levels. It works by dilating blood vessels, which can lead to a flushing sensation, hence it is sometimes referred to as “flushing niacin.” You can find detailed UV spectra of Niacin and information about its various lambda maxima by visiting the following link.

Nicotinamide, also known as niacinaminde, is a form of vitamin B₃ with the chemical formula C₆H₆N₂O₂. It is preferred treatment for pellagra and is a common treatment for acne due to it’s anti-inflammatory properties. If consumed daily, it is shown to decrease the risk of skin cancers other than melanoma. Nicotinamide is a precursor to nicotinamide adenine dinucleotide (NAD+), a coenzyme involved in various cellular processes, including energy metabolism. Nicotinamide is often used in skincare products for its anti-inflammatory properties and its ability to improve the appearance of aging skin and reduce hyperpigmentation. You can find detailed UV spectra of Nicotinamide and information about its various lambda maxima by visiting the following link.

Tranexamic acid, on the other hand, is a synthetic derivative of the amino acid lysine. It has the chemical formula C₈H₁₅NO₂. It is primarily used as an antifibrinolytic agent, meaning it helps to prevent the breakdown of blood clots. Tranexamic acid is commonly used to treat or prevent excessive bleeding, particularly in conditions such as heavy menstrual bleeding, traumatic hemorrhage, and during surgeries where significant blood loss is expected. Additionally, it has been investigated for its potential role in treating melasma, a common skin condition characterized by hyperpigmentation. You can find detailed UV spectra of Tranexamic acid and information about its various lambda maxima by visiting the following link.

Nicotinic Acid (Vitamin B3), Nicotinamide, Tranexamic acid can be retained and separated using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm