HPLC Method for Analysis of Thiamine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Vitamin B1 (Thiamine) on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin B1 (Thiamine)

Thiamine, also known as vitamin B1, is an essential nutrient that plays a crucial role in energy metabolism and the proper functioning of the nervous system. It helps the body convert carbohydrates into energy and is vital for the growth, development, and function of cells.

Vitamin B1 (Thiamine) can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.

You can find detailed UV spectra of Vitamin B1 (Thiamine) and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 10%
BufferH2SO4 -0.4%
Flow Rate1.0 ml/min
DetectionUV 250 nm
Samples1 mg/ml in H2O
Injection volume1 µl
LOD*60 ppb (250 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Vitamins
Analyzing CompoundsVitamin B1 (Thiamine)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Vitamin B1 (Thiamine)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.