HPLC LC MS Method for Analysis of Cyanocobalamin on Chromni Column

HPLC Method for Analysis of Cyanocobalamin on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cyanocobalamin.

Cyanocobalamin, also known as vitamin B12, is an essential nutrient with the chemical formula C63H88CoN14O14P. It is naturally present in some foods and available as a dietary supplement and as prescription medication. Vitamin B12 exists in several forms and contains the mineral cobalt, so compounds with vitamin B12 activity are collectively called “cobalamins”. It plays an essential role in the production of the red blood cells and DNA, as well as in the proper functioning of the nervous system. You can find detailed UV spectra of Cyanocobalamin and information about its various lambda maxima by visiting the following link.

Cyanocobalamin can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium acetate buffer. Detection is performed using LC MS.

Condition

ColumnChromni, 2.1 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseGradient MeCN/H2O – 70/30 – 50/50%, 10 min
BufferAmmonium acetate pH 5.0 – 20 mM
Flow Rate0.2 ml/min
DetectionESI-SIM: [M+2H]2+ 265.1
Limit Of Detection*10 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsVitamin
Analyzing CompoundsCyanocobalamin

Application Column

Chromni

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Cyanocobalamin
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.