HPLC Method for Analysis of Maleic acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid on BIST™ A+ Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Organic Acids on BIST™ A+ Column
HPLC Method for Analysis of Maleic acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid on BIST™ A+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Maleic  acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid

The maleate ion from Maleic acid is a popular ingredient as the maleate salt in several different drugs, including Methergine, Pyrilamine, and Carfenazine, among others. Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient for the human body and is sometimes taken as a treatment for high cholesterol. Aconitic acid is a key intermediary in the citric acid cycle, and is also used a flavoring agent and in the production of rubbers and plastics. Fumaric acid is a popular preservative and food additive with a fruit-like taste. Using SIELC’s newly introduced BIST™ method, a mixture of these organic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these organic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.



ColumnBIST™ A+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN Gradient
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) phosphate  – 5 mM pH 4.0
Flow Rate1.0 ml/min
DetectionUV 250 nm


Class of CompoundsAcids, Organic acid
Analyzing CompoundsMaleic  acid, Ascorbic acid, Nicotinic acid, Fumaric acid and Oxalic acid

Application Column


BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Ascorbic Acid
Fumaric Acid
Maleic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Oxalic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.