HPLC Separation Of Mixture of Pyridylacetic Acids And Their Derivatives

HPLC Method for 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide 

Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.

Nicotinic acid, also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is crucial for energy production.

Derrivatives are compounds formed when the parent compound goes through a chemical recation, replacing atoms with different ones, leading to different physical and chemical properties.

2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.

ColumnPrimesep 200, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O Gradient – 5-50%
BufferCH2O2 – 0.02-0.05%
Flow Rate1.0ml/min
DetectionUV, 270 nm
Class of Compounds
Acids
Analyzing Compounds2-Pyridylacetic acid hydrochloride, 3-Pyridylacetic acid hydrochloride, 4-Pyridylacetic acid hydrochloride, Nicotinic Acid/Niacin (3-pyridinecarboxylic acid), 3-Acetylpyridine, Pyridyl-3-thioacetomorpholide

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2-Pyridylacetic acid hydrochloride
3-Acetylpyridine
3-Pyridylacetic acid hydrochloride
4-Pyridylacetic acid hydrochloride
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Pyridyl-3-thioacetomorpholide

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.