HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column

Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).


Column Primesep S2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 85/15%
Buffer AmAc pH 5.0 15 mM
Flow Rate 1.0 ml/min
Detection ELSD, 50C UV 250 nm


Class of Compounds Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Toluenesulfonic acid, Naphthalenedisulfonic acid, 3.5 DHBA, Ascorbic acid,
Application Analytes:
1,5-Naphthalenedisulfonic Acid
3,5-Dihydroxybenzoic Acid
Ascorbic Acid
Organic Acids
p-Toluenesulfonic Acid (PTSA)