1,5-Naphthalenedisulfonic Acid

1,5-Naphthalenedisulfonic Acid structural formula

CAS Number81-04-9
Molecular FormulaC10H8O6S2
Molecular Weight288.291
InChI KeyXTEGVFVZDVNBPF-UHFFFAOYSA-N
LogP-0.262
Synonyms
  • 1,5-Naphthalenedisulfonic acid
  • Naphthalene-1,5-disulfonic acid
  • 81-04-9
  • 4-11-00-00561
  • BRN 0619838
  • EINECS 201-317-9
  • 1,5-Naphthylene disulfonic acid
  • NSC 9588
  • UNII-E8FPK78470
  • Armstrong's acid
  • naphthalene-1,5-disulfonic acid
  • 46874-44-6

Applications:

HPLC Method for Analysis of 2 – Naphthalenesulfonic acid and 1,5 -Naphthalenedisulfonic acid on BIST A Column

December 1, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of 2 – Naphthalenesulfonic acid and 1,5 -Naphthalenedisulfonic acid on BIST A Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  2 - Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid
HPLC Method for Analysis of 2 – Naphthalenesulfonic acid and 1,5 -Naphthalenedisulfonic acid

High Performance Liquid Chromatography (HPLC) Method for Analysis of  2 – Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid

2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, are two similarly-structured compounds. 2-Naphthalenesulfonic acid is commonly used in dye production and 1,5-Naphthalenedisulfonic acid is used in synthesizing the salts of basic drugs and in electrokinetic chromatography. Using SIELC’s newly introduced BIST™ method, these two acids can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid can be retained and UV detected at 270 nm.

Condition

ColumnBIST™ A, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 80/20%
BufferTMDAP formate pH 4.0 – 5,0 mM
Flow Rate1.0 ml/min
Detection270 nm

Description

Class of CompoundsAcid, Sulfonic acid
Analyzing Compounds2 – Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid

Application Column

BIST A

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
1,5-Naphthalenedisulfonic Acid
Naphthalenesulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom B Column

March 2, 2021


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1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge.  Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  The acid can be retained with great peak shape on a Newcrom BH mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.

Condition 1

Column Newcrom B, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV 230nm



 


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Condition 2

Column Newcrom B, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 70/30%
Buffer Ammonium Formate pH 3.0 – 40 mM
Flow Rate 0.5 ml/min
Detection UV 285 nm

Description

Class of Compounds Acid
Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
1,5-Naphthalenedisulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom BH Column

June 25, 2020


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1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge.  Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  The acid can be retained with great peak shape on a Newcrom BH mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.

Condition

Column Newcrom BH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer Ammonium Formate – 100 mM, pH 3.0
Flow Rate 0.5  ml/min
Detection UV 285 nm

Description

Class of Compounds Acid
Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
1,5-Naphthalenedisulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column

July 14, 2011

Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).

Condition

Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 85/15%
Buffer AmAc pH 5.0 15 mM
Flow Rate 1.0 ml/min
Detection ELSD, 50C UV 250 nm

 

Description

Class of Compounds
Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Toluenesulfonic acid, Naphthalenedisulfonic acid, 3.5 DHBA, Ascorbic acid,

 

Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
1,5-Naphthalenedisulfonic Acid
3,5-Dihydroxybenzoic Acid
Ascorbic Acid
Organic Acids
p-Toluenesulfonic Acid (PTSA)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns

July 16, 2009

Naphthalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.

Condition

Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Acid
Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
1,5-Naphthalenedisulfonic Acid
Sulfonic Acid

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.