HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom BH Column

View on hplc.cloud

1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge.  Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  The acid can be retained with great peak shape on a Newcrom BH mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.


Column Newcrom BH, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer Ammonium Formate – 100 mM, pH 3.0
Flow Rate 0.5  ml/min
Detection UV 285 nm


Class of Compounds Acid
Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
1,5-Naphthalenedisulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.