HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom BH Column

HPLC.cloud View on hplc.cloud 1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge.  Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  The acid can be retained with great peak shape on a Newcrom BH mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.


Column Newcrom BH, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer Ammonium Formate - 100 mM, pH 3.0
Flow Rate 0.5  ml/min
Detection UV 285 nm


Class of Compounds Acid
Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

Application Analytes:
1,5-Naphthalenedisulfonic Acid