Sulfonic Acid

Synonyms

    Applications:

    HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns

    July 16, 2009

    Naphthalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.

    Condition

    Column Obelisc N , 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection UV, 270 nm

     

    Description

    Class of Compounds
    Acid
    Analyzing Compounds 1,5-Naphthalenedisulfonic Acid

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    1,5-Naphthalenedisulfonic Acid
    Sulfonic Acid

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.