1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge. Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing. The acid can be retained with great peak shape on a Newcrom BH mixed-mode column. The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules. The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible. UV detection at 260nm can also be used.
| Column | Newcrom B, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 230nm |
| Column | Newcrom B, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 285 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 1,5-Naphthalenedisulfonic Acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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