HPLC Method for Analysis of 2 – Naphthalenesulfonic acid and 1,5 -Naphthalenedisulfonic acid on BIST A Column

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of 2 – Naphthalenesulfonic acid and 1,5 -Naphthalenedisulfonic acid on BIST A Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  2 - Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid
HPLC Method for Analysis of 2 – Naphthalenesulfonic acid and 1,5 -Naphthalenedisulfonic acid

High Performance Liquid Chromatography (HPLC) Method for Analysis of  2 – Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid

2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, are two similarly-structured compounds. 2-Naphthalenesulfonic acid is commonly used in dye production and 1,5-Naphthalenedisulfonic acid is used in synthesizing the salts of basic drugs and in electrokinetic chromatography. Using SIELC’s newly introduced BIST™ method, these two acids can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid can be retained and UV detected at 270 nm.

Condition

ColumnBIST™ A, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 80/20%
BufferTMDAP formate pH 4.0 – 5,0 mM
Flow Rate1.0 ml/min
Detection270 nm

Description

Class of CompoundsAcid, Sulfonic acid
Analyzing Compounds2 – Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid

Application Column

BIST A

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
1,5-Naphthalenedisulfonic Acid
Naphthalenesulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.