Primesep D mixed-mode column separates organic acids such as succinic acid, malic acid, MPS and butanesulfonate by a mixture of anion exchange and reversed phase mechanisms. Retention times can be changed by adjusting the percentage of acetonitrile in the mobile phase. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium formate as a buffer, making the method MS-compatible. Can also use UV detection at 250 nm.
SIELC Technologies usually develops more than one method for each compound
on our website. Therefore, this particular method may not be the best
available method from our portfolio for your specific application. Before
you decide to implement this method in your research, please send us an
email to email@example.com so we
can ensure you get optimal results for your compound of interest.