HPLC Method for Analysis of TFA (Trifluoroacetic Acid) on Primesep B4 by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of TFA (Trifluoroacetic Acid)

Trifluoroacetic acid (TFA) is a strong organic acid with the chemical formula CF₃COOH.

Properties:

• TFA is a colorless liquid at room temperature.
• It is a highly corrosive and volatile acid.
• It is miscible with common organic solvents.

Uses:

• Trifluoroacetic acid is commonly used in organic chemistry as a reagent, particularly in peptide synthesis and purification.
• It is used as a strong acid in various chemical reactions.
• TFA is also employed in the analysis of compounds by nuclear magnetic resonance (NMR) spectroscopy.

Precautions:

• Due to its corrosive nature, trifluoroacetic acid should be handled with care, and appropriate safety measures should be taken, including the use of protective equipment.

Trifluoroacetic acid can be retained and analyzed using an Primesep B4 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD

HPLC Method for TFA (Trifluoroacetic Acid), Acetic Acid, Chloroacetic acid, Dichloroacetic acid, Trichloroacetic acid, Difluoroacetic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of TFA (Trifluoroacetic Acid), Acetic Acid, Chloroacetic acid, Dichloroacetic acid, Trichloroacetic acid, Difluoroacetic acid.

Acetic Acid is the second simplest carboxylic acid with the chemical formula CH₃COOH. It is well known for being the active ingredient in vinegar, leading to the belief that it is the earliest mass produced acid, dating back to 3BC. Outside of it’s use in food and household matters, it is also used in production of vinyl acetate and cellulose acetate.

Chloroacetic Acid, also known as monochloroacetic acid (MCA), is a very toxic acid with the chemical formula ClCH₂CO₂H. It is most often used in the production of other chemicals such as phenoxy herbicides, carboxymethyl cellulose, and carboxymethyl starch. It is considered extremely hazardous as it can cause burns on skin and eyes as well as be fatal if inhaled or swallowed.

Difluoroacetic Acid (DFA) is a dihalogenocarboxylic acid with the chemical formula CHF₂COOH. As a solution, it quickly dissociates to form difluoroacetate ions.

Dichloroacetic Acid (DCA), also known as bichloroacetic acid (BCA), is a highly corrosive acid with the chemical formula C₂H₂Cl₂O₂. While it is used in personal care items and disinfectants, it is a known carcinogen.  Despite that, research shows that it may be a plausible treatment for certain cancers.

Trifluoroacetic Acid (TFA) is a synthetic organofluorine acid with the chemical formula C₂HF₃O₂. It is corrosive and toxic to aquatic life and mammals, causing severe irritation and burns to skin, eyes, and the respiratory tract. Not only that, TFA is also highly mobile and persistent, leading to high retention of it in soil and water. Determination of it’s threat level on the environmental and health levels are still ongoing.

Trichloroacetic Acid (TCA), also known as trichloroethanoic acid, is an analogue of acetic acid with the chemical formula C₂HCl₃O₂. It is often used as a skin peeling treatment to exfoliate damaged skin and encourage collagen production. In laboratory research, it is used for precipitating proteins and to extract and prepare standards for ascorbic acid (Vitamin C) assays.

Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis.  All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

HPLC Method for Trichloroacetic acid, Dichloroacetic acid, TFA (Trifluoroacetic Acid) on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Trichloroacetic acid, Dichloroacetic acid, TFA (Trifluoroacetic Acid).

Dichloroacetic Acid (DCA), also known as bichloroacetic acid (BCA), is a highly corrosive acid with the chemical formula C₂H₂Cl₂O₂. While it is used in personal care items and disinfectants, it is a known carcinogen.  Despite that, research shows that it may be a plausible treatment for certain cancers.

Trifluoroacetic Acid (TFA) is a synthetic organofluorine acid with the chemical formula C₂HF₃O₂. It is corrosive and toxic to aquatic life and mammals, causing severe irritation and burns to skin, eyes, and the respiratory tract. Not only that, TFA is also highly mobile and persistent, leading to high retention of it in soil and water. Determination of it’s threat level on the environmental and health levels are still ongoing.

Trichloroacetic Acid (TCA), also known as trichloroethanoic acid, is an analogue of acetic acid with the chemical formula C₂HCl₃O₂. It is often used as a skin peeling treatment to exfoliate damaged skin and encourage collagen production. In laboratory research, it is used for precipitating proteins and to extract and prepare standards for ascorbic acid (Vitamin C) assays.

Trichloroacetic acid, Dichloroacetic acid, TFA (Trifluoroacetic Acid) can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

HPLC Method for TFA (Trifluoroacetic Acid) on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of TFA (Trifluoroacetic Acid).

Trifluoroacetic Acid (TFA) is a synthetic organofluorine acid with the chemical formula C₂HF₃O₂. It is a substitution derivative of acetic acid, with fluorine atoms replacing the hydrogen atoms of the acetyl group. It is also a much stronger acid than acetic acid. TFA is widely used in organic synthesis as well as ion pairing reagent or buffer in HPLC. It is corrosive and toxic to aquatic life and mammals, causing severe irritation and burns to skin, eyes, and the respiratory tract. Not only that, TFA is also highly mobile and persistent, leading to high retention of it in soil and water. Determination of it’s threat level on the environmental and health levels are still ongoing.

TFA can be detected at low UV. Using Newcrom BH mixed-mode column, a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer can be used to retain TFA and UV detect it at 200nm.

Separation type: Liquid Chromatography Mixed-mode

Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.

Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.

Ionic liquid is an ionic compound which is liquid at room (or close to room) temperature. Most of the ionic liquids are in a dynamic equilibrium where at any time more than 99.99% of the liquid is made up of ionic, rather than molecular, species. Room-temperature ionic liquids consist of bulky cation (for example, substituted imidazolium) compounds. A wide range of anions is used as counter ions in ionic liquids: organic and inorganic anions such as chloride, iodide, tetrafluoroborate, hexafluorophosphate, bistriflimide, triflate, tosylate. Ionic liquids are widely used as solvents in organic reactions. When products are isolated from ionic liquids, they need to be analyzed for residual ionic liquid content.
Because both constituents of the ionic liquid are very different in terms of charge and hydrophobic properties, it is impossible to analyze entire ionic liquids by traditional chromatography. An effective and universal method for analysis of ionic liquids is developed on an Obelisc R HPLC column. Components on the ionic liquids are retained based on ionic and hydrophobic interactions. Obelisc R column has both positively and negatively charged ionic groups, making it possible to retain and separate cations and anions of ionic liquids on one column. Method can be used for quantitative of various ionic liquids containing organic and inorganic ions. Retention time of basic component can be effectively adjusted by pH, stronger anionic and hydrophobic counter-ions might require higher buffer concentration. Composition can be monitored by combination of UV and ELSD or by LC/MS.

Trifluoroacetic acid (TFA) is a strong carboxylic acid, with pKa of 0.3. TFA is widely used in chemistry as reagent and as counter-ion for basic drugs and other compounds. TFA is very polar in nature, and has low UV activity. It is often required to quantitate trifluoracetic acid in various formulations. HPLC method for determination of trifluoroacetic acid on Obelisc N column provides a viable alternative for quantitation of TFA. TFA is retained by ion-exchange mechanism on mixed-mode HILIC column. Relative comparison shows high loadability of the method. Obelisc N columns show much better peak shape and retention than traditional HPLC columns (Atlantis T3 from Waters). Perfect peak symmetry is achieved by adjusting amount of acetonitrile, buffer pH and buffer concentration. TFA is monitored by low UV (200 nm). Alternatively trifluoroacetic acid can be analyzed by ESLD with ammonium formate in the mobile phase. TFA forms non-volatile salt with ammonia, thus giving ability to quantitate it.

Dual functionality of Obelisc N column allows to retain and provide efficient peaks of strong carboxylic acids such as TFA. Simple UV quantitation and identification method became possible due to the unique properties of this stationary phase. In case of an RP separation poor retention and peak symmetry was obtained at a very low concentration of ACN. Sulfuric acid was used to suppress ionization of TFA to increase the hydrophobicity. In case of a typical HILIC column the phosphoric acid was used to increase ionization to make TFA more polar to enhance polar retention. The types of columns that do not have the mixed mode nature of Obelisc N can’t provide significant retention and efficiency.