Paraquat

Paraquat structural formula

CAS Number4685-14-7
Molecular FormulaC12H14N2
Molecular Weight186.258
InChI KeyINFDPOAKFNIJBF-UHFFFAOYSA-N
LogP-2.77
Synonyms
  • Paraquat
  • 1,1'-Dimethyl-4,4'-bipyridin-1-ium
  • 4,4'-Bipyridinium, 1,1'-dimethyl-
  • 4685-14-7
  • N,N'-Dimethyl-4,4'-bipyridinium
  • 1,1'-Dimethyl-4,4'-bipyridinium
  • 4,4'-Bipyridinium, 1,1'-dimethyl-
  • 1,1'-Dimethyl-4,4'-bipyridinium cation
  • 1,1'-Dimethyl-4,4'-dipyridinium
  • 1,1'-dimetil-4,4'-bipiridinio
  • Dimethyl viologen
  • Methyl viologen dication
  • Methyl viologen ion(2+)
  • Methyl viologen(2+)
  • N,N'-Dimethyl-4,4'-bipyridinium dication
  • Spraytop-graze
  • Starfire
  • Dextrone X
  • 1,1'-Dimethyl-4,4'-bipyridinium salt
  • 1,1'-Dimethyl-4,4'-bipyridyldiylium ion
  • N,N'-Dimethyl-gamma,gamma'-dipyridylium
  • EINECS 225-141-7
  • Methyl viologen (2+)
  • UNII-PLG39H7695
  • 1,1'-dimethyl-4,4'-bipyridyldiylium
  • paraquat dication
  • paraquat ion
  • 116047-10-0

Applications:

HPLC – MS Method for Analysis of Paraquat on Obelisc R Column

December 22, 2023

HPLC Method for Analysis of Paraquat on Obelisc R by SIELC Technologies

HPLC Method for Analysis of Paraquat on Obelisc R Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Paraquat

Paraquat is a widely used, non-selective herbicide known for its high efficacy in controlling a wide range of weeds. It has been an important tool in agriculture since its introduction in the mid-20th century. However, its use is accompanied by significant safety concerns due to its high toxicity.

Paraquat can be retained and analyzed on a Obelisc R mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%,
BufferAmmonium Formate pH 3.0-50 mM
Flow Rate0.2 ml/min
DetectionSIM 186 +, UV 259 nm
Spray Voltage:1.5 kV
Nebulizing gas:1.5 L/min
Drying gas:15 L/min
 DL temp:250 ˚C
Heat Block:400 ˚C

Class of Compounds
Pesticides
Analyzing CompoundsParaquat

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Paraquat

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Paraquat and Diquat on BIST B+ Column

November 30, 2022

HPLC Method for Analysis of Paraquat and Diquat on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Paraquat and Diquat on BIST B+ Column
HPLC Method for Analysis of Paraquat and Diquat on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Paraquat and Diquat

Paraquat and Diquat are two of the most popular herbicides on the market. With fairly similar structures and interactions with typical ion-exchange columns, they are usually extremely difficult to separate. Using SIELC’s newly introduced BIST™ method, Paraquat and Diquat, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged herbicide analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. What allows these two compounds to be separated using BIST™ is the slight difference in charge position between the two analytes. Since the analytes interact so close to the surface, the minor difference in charge distribution is magnified and therefore significantly affects each analyte’s retention ability. Using this new and unique analysis method, Paraquat and Diquat can be retained and UV detected at 250 nm.


Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 250 nm
Peak Retention Time4.7, 5.9 min

Description

Class of CompoundsHerbicides
Analyzing CompoundsParaquat and Diquat

Application Column

BIST B

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options

BIST B+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Diquat
Paraquat
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Simultaneous Determination of -Quat Herbicides on Obelisc R Column

September 22, 2022

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlormequat, Mepiquat, Paraquat, Diquat

HPLC Method for Simultaneous Determination of -Quat Herbicides on Obelisc R Column by SIELC Technologies
HPLC Method for Simultaneous Determination of -Quat Herbicides on Obelisc R Column by SIELC Technologies

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 40%
Buffer Ammonium Formate pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD 50C

Description

Class of Compounds Herbicides
Analyzing Compounds

Chlormequat, Mepiquat, Cl-, Br-, Paraquat, Diquat

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Chlormequat
Diquat
Mepiquat
Paraquat
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Herbicides Paraquat, Diquat and Glyphosate in One Run with LC/MS Compatible Conditions

December 5, 2013

 

There is a need to separate different herbicides and pesticides in one run with LC/MS compatible conditions. Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)

 

Condition

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer Gradient AmFm pH 3.0 – 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Diquat
Glyphosate
Paraquat

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Paraquat and Diquat Separation in Non-aqueous Mobile Phase

July 10, 2013

Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)

 

Condition

Column Sharc 1, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH – 90/10%
Buffer H2SO4 – 0.2
Flow Rate 3 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat

Application Column

SHARC 1

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Diquat
Paraquat

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Paraquat, Diguat, and Glyphosate on Obelisc R Column

July 3, 2013

 

Paraquat, diquat and glyphosate are three of most widely used herbicides in the world. Paraquat and diquat are very polar and very basic quaternary amines. Glyphosate is an aminophosphonic analog of glycine. It is very polar and acidic at most of the pH of the mobile phase. Since glyphosate and the quats have opposite charges no ion-pairing method can be developed for the mixture of basic and acidic herbicides. All three herbicides were separated on the Obelisc R tri-modal column. Paraquat and diquat are retained by a cation-exchange mechanism, and glyphosate is retained by weak reversed-phase and strong anion-exchange mechanisms. This method can be used for analysis of common herbicides in fruits, vegetables, ground water, drinking water and other matrices. Method is LC/MS compatible and can be used to determine trace levels of herbicides.

 

Condition

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer Gradient AmFm pH 3.0 – 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Chloride
Diquat
Glyphosate
Paraquat
Sodium

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Mixture of Paraquat and Diquat

October 6, 2010


Paraquat and diquat are two of most widely used herbicides in the world. Both compounds are toxic for humans and animals. Presence of paraquat and diquat in water is regulated by EPA. Paraquat and diquat have two quaternary amines, making them very polar molecules. Paraquat and diquat also have similar formula. Due to low hydrophobicity and highly basic properties, they show limited retention on reversed-phase columns and produce very poor peak shape due to the residual silanol interactions. Primesep AB and Obelisc R column retain and separate these two compounds with perfect peak shape.

Application Column

Primesep AB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Diquat
Paraquat

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation Paraquat and Diquat on Obelisc R

March 3, 2010


Paraquat and diquat are two of most widely used herbicides in the world. Both compounds are toxic for humans and animals. Presence of paraquat and diquat in water is regulated by EPA. Paraquat and diquat have two quaternary amines, making them very polar molecules. Paraquat and diquat also have similar formula. Due to low hydrophobicity and highly basic properties, they show limited retention on reversed-phase columns and produce very poor peak shape due to the residual silanol interactions. Obelisc R column retains and separates these two compounds with perfect peak shape. Method can be used for analysis of paraquat and diquat in soil, ground and drinking water, and other samples. Method allows to separate both compounds in one run without ion-pairing reagent.

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Diquat
Paraquat

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.