Separation of Herbicides Paraquat, Diquat and Glyphosate in One Run with LC/MS Compatible Conditions


There is a need to separate different herbicides and pesticides in one run with LC/MS compatible conditions. Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)



Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer Gradient AmFm pH 3.0 - 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound of interest.
Application Analytes:

Application Detection:
UV Detection
ELSD/MS Detection