HPLC Method for Mepiquat, Paraquat, Diquat, Chlormequat on Obelisc R by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mepiquat, Paraquat, Diquat, Chlormequat.
Chlormequat is a plant growth regulator with the chemical formula C5H13ClN. It works through inhibiting gibberellin biosynthesis, which leads to reduced cell elongation and thicker stalks.
Mepiquat is a plant growth regulator with the chemical formula C7H16N+. It is used primarily to control the growth rate and quality of the yield.
Paraquat is a widely used, non-selective herbicide known for its high efficacy in controlling a wide range of weeds. It has been an important tool in agriculture since its introduction in the mid-20th century. However, its use is accompanied by significant safety concerns due to its high toxicity.
Diquat is an organic dication that is used as a contact herbicide. It has the chemical formula C12H12Br2N2. It is considered moderately toxic and harmful if swallowed, inhaled, or absorbed through skin. It degrades slowly when in nature due to bonding strongly to minerals and organic particles. Due to that, it is no longer approved for use in the European Union.
Mepiquat, Paraquat, Diquat, Chlormequat can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 40% |
| Buffer | Ammonium Formate pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Herbicides |
| Analyzing Compounds | Mepiquat, Paraquat, Diquat, Chlormequat |
Application Column
Obelisc R
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Diquat
Mepiquat
Paraquat
