HPLC Method for Analysis of Paraquat and Diquat on BIST B+ by SIELC Technologies.
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analyses of Paraquat and Diquat
Paraquat and Diquat are two of the most popular herbicides on the market. With fairly similar structures and interactions with typical ion-exchange columns, they are usually extremely difficult to separate. Using SIELC’s newly introduced BIST™ method, Paraquat and Diquat, which protonate in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged herbicide analytes to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. What allows these two compounds to be separated using BIST™ is the slight difference in charge position between the two analytes. Since the analytes interact so close to the surface, the minor difference in charge distribution is magnified and therefore significantly affects each analyte’s retention ability. Using this new and unique analysis method, Paraquat and Diquat can be retained and UV detected at 250 nm.
Condition
| Column | BIST B+, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 70% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Peak Retention Time | 4.7, 5.9 min |
Description
| Class of Compounds | Herbicides |
| Analyzing Compounds | Paraquat and Diquat |
Application Column
BIST B
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select options
BIST B+
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsApplication Analytes:
DiquatParaquat
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
