HPLC Method for Analysis of Paraquat on Obelisc R by SIELC Technologies
Paraquat is a widely used, non-selective herbicide with the chemical formula C12H14N2. It is known for its high efficacy in controlling a wide range of weeds. It has been an important tool in agriculture since its introduction in the mid-20th century. However, its use is accompanied by significant safety concerns due to its high toxicity. You can find detailed UV spectra of Paraquat and information about its various lambda maxima by visiting the following link.
Paraquat can be retained and analyzed on a Obelisc R mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
| Column | Obelisc R, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 50%, |
| Buffer | Ammonium Formate pH 3.0-50 mM |
| Flow Rate | 0.2 ml/min |
| Detection | SIM 186 +, UV 259 nm |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
| Class of Compounds | Pesticides |
| Analyzing Compounds | Paraquat |
Application Column
Obelisc R
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
