HPLC – MS Method for Analysis of Paraquat on Obelisc R Column

HPLC Method for Analysis of Paraquat on Obelisc R by SIELC Technologies

HPLC-MS-Method-for-Analysis-of-Diquat-on-Obelisc-R-Column
HPLC Method for Analysis of Paraquat on Obelisc R Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Paraquat

Paraquat is a widely used, non-selective herbicide known for its high efficacy in controlling a wide range of weeds. It has been an important tool in agriculture since its introduction in the mid-20th century. However, its use is accompanied by significant safety concerns due to its high toxicity.

Paraquat can be retained and analyzed on a Obelisc R mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%,
BufferAmmonium Formate pH 3.0-50 mM
Flow Rate0.2 ml/min
DetectionSIM 185 +, UV 259 nm
Spray Voltage:1.5 kV
Nebulizing gas:1.5 L/min
Drying gas:15 L/min
 DL temp:250 ˚C
Heat Block:400 ˚C

Class of Compounds
Pesticides
Analyzing CompoundsParaquat

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Paraquat

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.