Chlortetracycline

Chlortetracycline structural formula

CAS Number57-62-5
Molecular FormulaC22H23ClN2O8
Molecular Weight478.881
InChI KeyCYDMQBQPVICBEU-XRNKAMNCSA-N
LogP-0.620
Synonyms
  • Chlortetracycline
  • (4S,4aS,5aS,6S,12aS)-7-Chloro-4-(dimethylamino)-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aS,5aS,6S,12aS)-
  • 57-62-5
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamin o)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pe ntahydroxy-6-methyl-1,11-dioxo-, (4S-(4.alpha.,4a. alpha.,5a.alpha.,6.beta.,12a.alpha.))-
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aS,5aS,6S,12aS)-
  • [4S-(4α,4aα,5aα,6β,12aα)]-7-Chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-Octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, [4S-(4α,4aα,5aα,6β,12aα)]-
  • 7-Chlorotetracycline
  • 7-Chlortetracycline
  • Acronize
  • Acronize PD
  • Aureocarmyl
  • Aureociclin
  • Aureocina
  • Aureomicina
  • Aureomycin
  • Aureomykoin
  • Aurofac
  • Biomycin
  • Biomycin a
  • Centraureo
  • Chlortetracyclin
  • Chrusomykine
  • Chrysomykine
  • clortetraciclina
  • Duomycin
  • Flamycin
  • Orospray
  • Tri-chlortetracycline
  • Uromycin
  • Aureomycin A-377
  • Biomitsin
  • Caswell No. 219B
  • EINECS 200-341-7
  • EPA Pesticide Chemical Code 006301
  • Chlortetracyclinum
  • UNII-WCK1KIQ23Q
  • Chlormax
  • 103651-17-8
  • 1236363-43-1
  • 856305-72-1
  • 96066-24-9
  • 96968-60-4
  • 98271-27-3

Applications:

HPLC Method for Analysis of Chlortetracycline on Primesep 100 Column

November 12, 2026

HPLC Method for Chlortetracycline on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Chlortetracycline on Primesep 100 Column by SIELC Technologies

Chlortetracycline is a tetracycline antibiotic with the formula C22H23ClN2O8. It is used primarily in combination with triamcinolone to treat infected allergic dermatitis. It is used widely in veterinary medicine to treat conjunctivitis, infected wounds, and respiratory tract infections.

Chlortetracycline can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and H2SO4 as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4  – 0.1%
Flow Rate1 ml/min
DetectionUV 268 nm
LOD*60 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsMedication
Analyzing CompoundsChlortetracycline

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Chlortetracycline
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Antibiotics in Mixed-mode chromatography

May 11, 2015

 

A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).

 

Condition
Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 0-25%, 6 min, 25-70% 14 min
Buffer Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode

May 11, 2015

Oxytetracycline and chlortetracycline are polar compounds related to broad-spectrum of tetracycline group of antibiotics. Slight hydrophobicity and presence of the basic group in both compounds allow to employ mixed-mode reversed-phase cation-exchange mechanism for separation of these compounds. Method can be used for analysis of antibiotics and related impurities with LC/MS compatible conditions.

Condition
Column Primesep 100, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Oxytetracycline, Chlortetracycline

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Chlortetracycline
Oxytetracycline
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.