Carboxymethylcysteine

Carboxymethylcysteine

Synonyms

    Applications:

    Separation of Antibiotics in Mixed-mode chromatography

    2015-05-11
      A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).  

    Condition

    Column Obelisc R, 2.1x150 mm, 5 µm, 100A
    Mobile Phase Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
    Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold
    Flow Rate 1.0 ml/min
    Detection UV, 270 nm
     

    Description

    Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
    Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine
     
    Application Analytes:
    Carboxymethylcysteine
    Chlortetracycline
    Dapsone
    Flumequine
    Josamycin
    Marbofloxacin
    Norfloxacin
    Oxytetracycline
    Spiramycin
    Sulfamethoxazole
    Sulfonamides
    Sulphadiazine
    Sulphaguanidine
    Sulphanilamide
    Sulphaquinoxaline
    Tylosin

    Application Detection:
    UV Detection
    ELSD/MS Detection