Sulfonamides

111

Synonyms

    Applications:

    Separation of Antibiotics in Mixed-mode chromatography

    May 11, 2015

     

    A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).

     

    Condition

    Column Obelisc R, 2.1×150 mm, 5 µm, 100A
    Mobile Phase Gradient MeCN – 0-25%, 6 min, 25-70% 14 min
    Buffer Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold
    Flow Rate 1.0 ml/min
    Detection UV, 270 nm

     

    Description

    Class of Compounds
     Antibiotic, Drug, Hydrophobic, Ionizable
    Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine

     

    Application Column

    Obelisc R

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Carboxymethylcysteine
    Chlortetracycline
    Dapsone
    Flumequine
    Josamycin
    Marbofloxacin
    Norfloxacin
    Oxytetracycline
    Spiramycin
    Sulfamethoxazole
    Sulfonamides
    Sulphadiazine
    Sulphaguanidine
    Sulphanilamide
    Sulphaquinoxaline
    Tetracycline
    Tylosin

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

    May 5, 2005

    Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Benzenesulfonamide
    Chlorobenzenesulfonamide
    Hydroxybenzenesulfonamide
    Hydroxysulphaminophenylhydrazine
    Sulfanilamide
    Sulfonamides

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.