| CAS Number | 70458-96-7 |
|---|---|
| Molecular Formula | C16H18FN3O3 |
| Molecular Weight | 319.336 |
| InChI Key | OGJPXUAPXNRGGI-UHFFFAOYSA-N |
| LogP | -1.03 |
| Synonyms |
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Applications:
HPLC Retention Control of Antibiotics (Metronidazole and Norfloxacine) in Toothpaste
May 27, 2015
Commercially available toothpaste has been known to include antibiotics. To demonstrate the retention control on the mixed-mode column Primesep 100, toothpaste was spiked with the antibiotics metronidazole and norfloxacin. In a 13 minute method, retention of the antibiotics was controlled by adjustment in ratio of organic in the mobile phase. This method demonstrates the flexibility of using mixed-mode columns such as Primesep 100.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Antibiotics, Hydrophilic, Ionizable |
| Analyzing Compounds | Metronidazole, Norfloxacine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsNorfloxacin
Analysis of Antibiotic Norfloxacin and Related Impurities in Mixed-Mode Chromatography
May 12, 2015
The antibiotic norfloxacin is a first generation fluoroquinolone. It is effective against both gram positive and gram negative bacteria. Analysis was done on Obelisc R and Primesep D.
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-30%, 10 min, 5 min hold |
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 5 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Norfloxacin |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography
May 12, 2015
Separation type: Liquid Chromatography Mixed-mode
Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-50%, 10 min, 3 min hold |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Josamycin, Norfloxacin, Marbofloxacin, Flumequine |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsJosamycin
Marbofloxacin
Norfloxacin
UV Detection
Separation of Antibiotics in Mixed-mode chromatography
May 11, 2015
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 0-25%, 6 min, 25-70% 14 min |
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsChlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin
UV Detection
