HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography

Separation type: Liquid Chromatography Mixed-mode

Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.

Condition 2

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%, 10 min, 3 min hold
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds  Josamycin, Norfloxacin, Marbofloxacin, Flumequine
Application Analytes: