HPLC Separation of Antibiotics of Group in Mixed-Mode Chromatography

Separation type: Liquid Chromatography Mixed-mode

Primesep D was used to separate the antibiotics marbofloxacin, norfloxacin, josamycin, and flumequine. Primesep D is a reverse-phase column with embedded basic ion-pairing groups designed for analysis of plasma. This method is compatible with LC/MS and prep chromatography.

Condition 2

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 5-50%, 10 min, 3 min hold
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm



Class of Compounds
 Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds  Josamycin, Norfloxacin, Marbofloxacin, Flumequine


Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.