Sulfanilamide

CAS Number63-74-1
Molecular FormulaC6H8N2O2S
Molecular Weight172.202
InChI KeyFDDDEECHVMSUSB-UHFFFAOYSA-N
LogP-0.6
Synonyms
  • sulfanilamide
  • 4-Aminobenzenesulfonamide
  • 63-74-1
  • Sulphanilamide
  • Sulfamine
  • Sulphonamide
  • p-Aminobenzenesulfonamide
  • Sulfonylamide
  • p-Aminobenzenesulfamide
  • Bacteramid
  • Streptasol
  • p-Sulfamoylaniline
  • Prontosil Album
  • Sulfonamide P
  • Estreptocida
  • Exoseptoplix
  • Streptoclase
  • p-Sulfamidoaniline
  • Streptocide
  • Sulfamidyl
  • Sulfanalone
  • Sulfanidyl
  • Sulfanil
  • Sulfocidine
  • Sulfana
  • Sulfanilimidic acid
  • p-Anilinesulfonamide
  • p-Aminophenylsulfonamide
  • Ambeside
  • Antistrept
  • Astreptine
  • Astrocid
  • Bactesid
  • Collomide
  • Colsulanyde
  • Copticide
  • Deseptyl
  • Ergaseptine
  • Erysipan
  • Gombardol
  • Lysococcine
  • Neococcyl
  • Orgaseptine
  • Prontalbin
  • Prontylin
  • Proseptal
  • Proseptine
  • Proseptol
  • Pysococcine
  • Septanilam
  • Septinal
  • Septolix
  • Septoplex
  • Septoplix
  • Strepamide
  • Strepsan
  • Streptagol
  • Streptamid
  • Streptamin
  • Streptocid
  • Streptocom
  • Strepton
  • Streptopan
  • Streptosil
  • Streptozol
  • Streptozone
  • Streptrocide
  • Sulfocidin
  • Therapol
  • 4-Sulfamoylaniline
  • Albexan
  • Albosal
  • Dipron
  • Gerison
  • Infepan
  • Sanamid
  • Stramid
  • Tolder
  • Benzenesulfonamide, 4-amino-
  • Lusil
  • 4-Aminobenzene-1-Sulfonamide
  • Prontosil White
  • Pronzin Album
  • Septamide Album
  • Stopton Album
  • Streptocid album
  • Prontosil I
  • Rubiazol A
  • White streptocide
  • PABS

Applications:

HPLC Separation of Antibiotics

2020-06-11


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Antibiotics are widely used for treatment and prevention of bacterial infections.  Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups.  In addition, methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

Condition 1
Column Primesep 200, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN Gradient
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 275 nm

Condition 2
Column Newcrom A, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeOH Gradient
Buffer H2SO4 Gradient
Flow Rate  0.5 ml/min
Detection UV 275 nm

 

Description
Class of Compounds Drugs, Antibiotics
Analyzing Compounds Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline

Application Column

Primesep 200

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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Newcrom A

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.

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Application Analytes:
Dapsone
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Benzenesulfonamide and Sulfanilamide

2008-08-22


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Condition 1
Column Newcrom А, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeOH/H2O – 80/20%
Buffer H2SO4 – 0.005%
Flow Rate 0.5 ml/min
Detection  275 nm

Condition 2
Column Primesep D, , Primesep 100 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer Formic Acid – 0.1%
Flow Rate 0.5 ml/min
Detection UV 230 nm

Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options

Primesep D

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Benzenesulfonamide
Sulfanilamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Aromatic Sulfonamides and Hydrozine

2007-12-06

Application Analytes:
Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

2005-05-05

Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Sulfonamides
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.