HPLC Method for Sulfanilamide, Sulfadiazine, Dapsone, Sulfaguanidine, Sulphaquinoxaline on Newcrom A by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfanilamide, Sulfadiazine, Dapsone, Sulfaguanidine, Sulphaquinoxaline.

Antibiotics are widely used for treatment and prevention of bacterial infections. 

Sulfanilamide is a sulfonamide antibacterial drug with the chemical formula C₆H₈N₂O₂S. It’s height of use was during World War II to treat and prevent the spread of infections among the Allies. Due to later discovery of more effective antibiotics, it is no longer as widely used. You can find detailed UV spectra of Sulfanilamide and information about its various lambda maxima by visiting the following link.

Sulfadiazine is a sulfonamide antibiotic with the chemical formula C₁₀H₁₀N₄O₂S. It is the treatment of choice for toxoplasmosis and is considered the second-line treatment against numerous other infections.  It works by blocking the synthesis of folic acid in bacteria, which prevents cell reproduction. Sulfadiazine can be either taken orally or applied topically. You can find detailed UV spectra of Sulfadiazine and information about its various lambda maxima by visiting the following link.

Sulfaguanidine is a sulfonamide antibiotic with the chemical formula C₇H₁₀N₄O₂S. It is a guanidine derivative of sulfanilamide that works through inhibiting the synthesis of folic acid in bacteria. Most often, it is used to treat Bacillary dysentery.

Dapsone is a sulfone antibiotic with anti-inflammatory properties. As a gel, it is sold under the brand name Aczone as acne treatment, but it can also be used as part of treatment for other skin conditions including leprosy and dermatitis herpetiformis. It’s chemical formula is C₁₂H₁₂N₂O₂S.

Sulfaquinoxaline is a sulfonamide antibiotic that is typically used in veterinary medicine. It is used to treat Coccidiosis in cattle and sheep, as well as a variety infections in poultry. It is deemed not suitable for human use. It’s chemical formula is C₁₄H₁₂N₄O₂S.

Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

HPLC Method for Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone.

Sulfanilamide is a sulfonamide antibacterial drug with the chemical formula C₆H₈N₂O₂S. It’s height of use was during World War II to treat and prevent the spread of infections among the Allies. Due to later discovery of more effective antibiotics, it is no longer as widely used. You can find detailed UV spectra of Sulfanilamide and information about its various lambda maxima by visiting the following link.

Sulfadiazine is a sulfonamide antibiotic with the chemical formula C₁₀H₁₀N₄O₂S. It is the treatment of choice for toxoplasmosis and is considered the second-line treatment against numerous other infections.  It works by blocking the synthesis of folic acid in bacteria, which prevents cell reproduction. Sulfadiazine can be either taken orally or applied topically. You can find detailed UV spectra of Sulfadiazine and information about its various lambda maxima by visiting the following link.

Sulfaguanidine is a sulfonamide antibiotic with the chemical formula C₇H₁₀N₄O₂S. It is a guanidine derivative of sulfanilamide that works through inhibiting the synthesis of folic acid in bacteria. Most often, it is used to treat Bacillary dysentery.

Dapsone is a sulfone antibiotic with anti-inflammatory properties. As a gel, it is sold under the brand name Aczone as acne treatment, but it can also be used as part of treatment for other skin conditions including leprosy and dermatitis herpetiformis. It’s chemical formula is C₁₂H₁₂N₂O₂S.

Sulfaquinoxaline is a sulfonamide antibiotic that is typically used in veterinary medicine. It is used to treat Coccidiosis in cattle and sheep, as well as a variety infections in poultry. It is deemed not suitable for human use. It’s chemical formula is C₁₄H₁₂N₄O₂S.

Antibiotics are widely used for treatment and prevention of bacterial infections.  Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups.  In addition, methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

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Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.