| CAS Number | 68-35-9 |
|---|---|
| Molecular Formula | C10H10N4O2S |
| Molecular Weight | 260.28 |
| InChI Key | SEEPANYCNGTZFQ-UHFFFAOYSA-N |
| LogP | -0.1 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Sulfadiazine
July 19, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Antibiotics on Newcrom A Column
June 11, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
| Column | Newcrom A, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH Gradient |
| Buffer | H2SO4 Gradient |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Newcrom A
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline
HPLC Separation of Antibiotics on Primesep 200 Column
June 6, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups. In addition, methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
| Column | Primesep 200, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN Gradient |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Sulfadiazine
Sulfaguanidine
Sulfanilamide
HPLC Method for Analysis of Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate
July 11, 2017
Sulfadiazine is an antibiotic used to prevent rheumatic fever, chancroid, chlamydia and infections by Haemophilus influenzae. There are side effects to the use of the drug which include, but not limited to: nausea, headache, rash, depression.
| Column | Primesep 100, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 20-75%, 4 min |
| Buffer | Gradient H3PO4 – 0.05-0.2%, 4 min |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsDexamethasone
Naphthylthiourea
Sulfadiazine
Sulfamethazine
