HPLC Separation of Antibiotics on Newcrom A Column

HPLC Method for Sulfanilamide, Sulfadiazine, Dapsone, Sulfaguanidine, Sulphaquinoxaline on Newcrom A by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfanilamide, Sulfadiazine, Dapsone, Sulfaguanidine, Sulphaquinoxaline.

Antibiotics are widely used for treatment and prevention of bacterial infections. 

Sulfanilamide is a sulfonamide antibacterial drug with the chemical formula C6H8N2O2S. It’s height of use was during World War II to treat and prevent the spread of infections among the Allies. Due to later discovery of more effective antibiotics, it is no longer as widely used. You can find detailed UV spectra of Sulfanilamide and information about its various lambda maxima by visiting the following link.

Sulfadiazine is a sulfonamide antibiotic with the chemical formula C10H10N4O2S. It is the treatment of choice for toxoplasmosis and is considered the second-line treatment against numerous other infections.  It works by blocking the synthesis of folic acid in bacteria, which prevents cell reproduction. Sulfadiazine can be either taken orally or applied topically. You can find detailed UV spectra of Sulfadiazine and information about its various lambda maxima by visiting the following link.

Sulfaguanidine is a sulfonamide antibiotic with the chemical formula C7H10N4O2S. It is a guanidine derivative of sulfanilamide that works through inhibiting the synthesis of folic acid in bacteria. Most often, it is used to treat Bacillary dysentery.

Dapsone is a sulfone antibiotic with anti-inflammatory properties. As a gel, it is sold under the brand name Aczone as acne treatment, but it can also be used as part of treatment for other skin conditions including leprosy and dermatitis herpetiformis. It’s chemical formula is C12H12N2O2S.

Sulfaquinoxaline is a sulfonamide antibiotic that is typically used in veterinary medicine. It is used to treat Coccidiosis in cattle and sheep, as well as a variety infections in poultry. It is deemed not suitable for human use. It’s chemical formula is C14H12N4O2S.

Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups.  Methanol can be used in the mobile phase on Newcrom A column.  The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer.  UV detection at 275nm.

Condition

Column Newcrom A, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeOH Gradient
Buffer H2SO4 Gradient
Flow Rate  0.5 ml/min
Detection UV 275 nm

 

Description

Class of Compounds Drugs, Antibiotics
Analyzing Compounds Sulfanilamide, Sulfadiazine, Dapsone, Sulfaguanidine, Sulphaquinoxaline

Application Column

Newcrom A

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Dapsone
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.