Cortisone 21-Acetate

CAS Number50-04-4
Molecular FormulaC23H30O6
Molecular Weight402.5
InChI KeyITRJWOMZKQRYTA-RFZYENFJSA-N
LogP2.1
Synonyms
  • CORTISONE ACETATE
  • Cortisone 21-acetate
  • 50-04-4
  • Cortone acetate
  • Incortin
  • Biocort acetate
  • Artriona
  • Cortadren
  • Scheroson
  • Cortisyl
  • Adreson
  • Irisone acetate
  • Cortelan
  • Cortistab
  • Ricortex
  • Corlin
  • Cortogen acetate
  • Compound E acetate
  • Cortisone (acetate)
  • Cortisone monoacetate
  • NSC 49420
  • Cortisone, 21-acetate
  • Pregn-4-ene-3,11,20-trione, 21-(acetyloxy)-17-hydroxy-
  • Cortilen
  • UNII-883WKN7W8X
  • 11-Dehydro-17-hydroxycorticosterone-21-acetate
  • CCRIS 3661
  • 11-Dehydro-17-hydroxycorticosterone acetate
  • EINECS 200-006-5
  • 17,21-Dihydroxypregn-4-ene-3,11,20-trione 21-acetate
  • 883WKN7W8X
  • CHEBI:3897
  • Pregn-4-ene-3,11,20-trione, 17,21-dihydroxy-, 21-acetate
  • ITRJWOMZKQRYTA-RFZYENFJSA-N
  • MFCD00003609
  • 21-(Acetyloxy)-17-hydroxypregn-4-ene-3,11,20-trione
  • 21-Acetoxy-17,alpha-hydroxy-3,11,20-triketopregnene-4
  • 21-Acetoxy-17,alpha-hydroxypregn-4-ene-3,11,20-trione
  • NCGC00021191-07
  • 4-Pregnene-17,alpha,21-diol-3,11,20-trione 21-acetate
  • DSSTox_CID_2858
  • DSSTox_RID_76761
  • DSSTox_GSID_22858
  • 17-Hydroxy-3,11,20-trioxopregn-4-en-21-yl acetate
  • 21-Acetoxy-4-pregnen-17alpha-ol-3,11,20-trione
  • Cortisone 21-acetate, 98+%
  • 17,21-Dihydroxypregn-4-ene-3,11,20-trione-21-acetate
  • 17alpha,21-dihydroxypregn-4-ene-3,11,20-trione acetate
  • CAS-50-04-4
  • [2-[(8S,9S,10R,13S,14S,17R)-17-hydroxy-10,13-dimethyl-3,11-dioxo-1,2,6,7,8,9,12,14,15,16-decahydrocyclopenta[a]phenanthren-17-yl]-2-oxo-ethyl] acetate
  • [2-[(8S,9S,10R,13S,14S,17R)-17-hydroxy-10,13-dimethyl-3,11-dioxo-1,2,6,7,8,9,12,14,15,16-decahydrocyclopenta[a]phenanthren-17-yl]-2-oxoethyl] acetate
  • 2-((1S,10S,11S,15S,2R,14R)-14-hydroxy-2,15-dimethyl-5,17-dioxotetracyclo[8.7.0 .0<2,7>.0<11,15>]heptadec-6-en-14-yl)-2-oxoethyl acetate
  • 2-((8S,9S,10R,13S,14S,17R)-17-hydroxy-10,13-dimethyl-3,11-dioxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl acetate
  • SMR000059124
  • Cortisone acetate (Cortone)
  • Cortisone acetate [USP:JAN]
  • Cortisone acetate [USAN:JAN]
  • Acetate cortisone
  • Cortisyl artriona
  • component of Neosone
  • Neosone (Salt/Mix)
  • Cortisone-21-acetate
  • Cortone acetate (TN)
  • Cortisone-acetate-Cortone
  • AC1L1L1N
  • AC1Q62MF
  • SCHEMBL4501
  • CHEMBL1650
  • Lopac0_000271
  • MLS000069478
  • MLS002207136
  • MLS002548859
  • ARONIS24358
  • Cortisone acetate (JP17/USP)
  • DTXSID0022858
  • CTK8D6790
  • Cortisone 21-acetate, >=99%
  • HMS2235D24
  • HMS3259N19
  • HMS3260H04
  • BCP08487
  • NSC49420
  • ZINC3875334
  • Tox21_113482
  • Tox21_500271
  • BDBM50455157
  • HY-17461A
  • LMST02030120
  • NSC-49420
  • SBB080776
  • AKOS005267225
  • Tox21_113482_1
  • AC-6827
  • AN-9606
  • CCG-204366
  • CS-1742
  • DB01380
  • LP00271
  • MCULE-8390750975
  • NC00677
  • NCGC00021191-03
  • NCGC00021191-06
  • NCGC00021191-08
  • NCGC00021191-09
  • NCGC00260956-01
  • AK163492
  • AS-13692
  • CC-25981
  • LS-55003
  • ST075179
  • AB2000655
  • EU-0100271
  • FT-0665176
  • S2559
  • ST24046370
  • C08173
  • D00973
  • J10115
  • AB00384283-08
  • AB00384283_09
  • 003C609
  • C-21758
  • W-105993
  • BRD-K86161929-001-13-3
  • Pregn-4-ene-3,20-trione, 21-(acetyloxy)-17-hydroxy-
  • 17-Hydroxy-3,11,20-trioxopregn-4-en-21-yl acetate
  • #21-Acetoxy-17.alpha.-hydroxy-3,11,20-triketopregnene-4
  • 21-Acetoxy-17.alpha.-hydroxypregn-4-ene-3,11,20-trione
  • 4-Pregnen-17.alpha.,21-diol-3,11,20-trione 21-acetate
  • 4-Pregnene-17alpha,21-diol-3,11,20-trione 21-acetate
  • Pregn-4-ene-3,20-trione, 17,21-dihydroxy-, 21-acetate
  • 17alpha,21-Dihydroxy-4-pregnene-3,11,20-trione 21-acetate
  • 4-Pregnene-17.alpha.,21-diol-3,11,20-trione 21-acetate
  • Cortisone acetate, European Pharmacopoeia (EP) Reference Standard
  • Cortisone acetate, United States Pharmacopeia (USP) Reference Standard

Applications:

HPLC Method for Analysis of Cortisone 21-Acetate on Primesep 100 Column

August 2, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cortisone 21-Acetate on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

Cortisone 21-Acetate
HPLC Method for Analysis of Cortisone 21-Acetate on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Cortisone 21-Acetate

Cortisone 21-acetate is a synthetic corticosteroid used in the treatment of various inflammatory and autoimmune conditions. It is the acetate ester of cortisone, which increases its stability and bioavailability when administered.

Mechanism of Action

Cortisone 21-acetate is a prodrug of cortisone, which is converted to its active form, cortisol, in the liver. Cortisol exerts its effects by binding to glucocorticoid receptors, which modulate the expression of anti-inflammatory proteins and inhibit the expression of pro-inflammatory proteins.

Uses

  • Anti-inflammatory: Used to treat conditions such as arthritis, lupus, psoriasis, ulcerative colitis, and allergic reactions.
  • Immunosuppressive: Employed in the management of autoimmune diseases to suppress the immune response.
  • Hormone Replacement Therapy: Used in the treatment of adrenal insufficiency and Addison’s disease.

Cortisone 21-Acetate can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of Cortisone 21-Acetate and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH3PO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 250 nm
Samples1.0 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*10 ppb (250 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Synthetic corticosteroid
Analyzing CompoundsCortisone 21-Acetate

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Cortisone 21-Acetate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Cortisone 21-Acetate

July 19, 2024

UV-Vis Spectrum of Cortisone 21-Acetate. Absorption Maxima: 242 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Cortisone 21-Acetate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate

July 11, 2017

Sulfadiazine is an antibiotic used to prevent rheumatic fever, chancroid, chlamydia and infections by Haemophilus influenzae. There are side effects to the use of the drug which include, but not limited to: nausea, headache, rash, depression.

 

Condition
Column Primesep 100, 3.2×50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 20-75%, 4 min
Buffer Gradient H3PO4 – 0.05-0.2%, 4 min
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cortisone 21-Acetate
Dexamethasone
Naphthylthiourea
Sulfadiazine
Sulfamethazine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.