HPLC Method for Analysis of Cortisone 21-Acetate on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cortisone 21-Acetate on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

Cortisone 21-Acetate
HPLC Method for Analysis of Cortisone 21-Acetate on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Cortisone 21-Acetate

Cortisone 21-acetate is a synthetic corticosteroid used in the treatment of various inflammatory and autoimmune conditions. It is the acetate ester of cortisone, which increases its stability and bioavailability when administered.

Mechanism of Action

Cortisone 21-acetate is a prodrug of cortisone, which is converted to its active form, cortisol, in the liver. Cortisol exerts its effects by binding to glucocorticoid receptors, which modulate the expression of anti-inflammatory proteins and inhibit the expression of pro-inflammatory proteins.

Uses

  • Anti-inflammatory: Used to treat conditions such as arthritis, lupus, psoriasis, ulcerative colitis, and allergic reactions.
  • Immunosuppressive: Employed in the management of autoimmune diseases to suppress the immune response.
  • Hormone Replacement Therapy: Used in the treatment of adrenal insufficiency and Addison’s disease.

Cortisone 21-Acetate can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of Cortisone 21-Acetate and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH3PO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 250 nm
Samples1.0 mg/ml in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*10 ppb (250 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Synthetic corticosteroid
Analyzing CompoundsCortisone 21-Acetate

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Cortisone 21-Acetate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.