Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode

Oxytetracycline and chlortetracycline are polar compounds related to broad-spectrum of tetracycline group of antibiotics. Slight hydrophobicity and presence of the basic group in both compounds allow to employ mixed-mode reversed-phase cation-exchange mechanism for separation of these compounds. Method can be used for analysis of antibiotics and related impurities with LC/MS compatible conditions.

Condition
Column Primesep 100, 4.6×150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Oxytetracycline, Chlortetracycline

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Chlortetracycline
Oxytetracycline
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.