HPLC Method for Analysis of Chlortetracycline on Primesep 100 Column

HPLC Method for Chlortetracycline on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Chlortetracycline on Primesep 100 Column by SIELC Technologies

Chlortetracycline is a tetracycline antibiotic with the formula C22H23ClN2O8. It is used primarily in combination with triamcinolone to treat infected allergic dermatitis. It is used widely in veterinary medicine to treat conjunctivitis, infected wounds, and respiratory tract infections.

Chlortetracycline can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and H2SO4 as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4  – 0.1%
Flow Rate1 ml/min
DetectionUV 268 nm
LOD*60 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsMedication
Analyzing CompoundsChlortetracycline

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Chlortetracycline
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.