Separation type: Liquid Chromatography Mixed-mode

Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Glutaric acid is a common plasticizer precursor and often used in the production of polyester polyols and polyamides. Interestingly, it is significantly more water soluble than either Succinic or Adipic acid. Adipic acid, also known as hexanedioic acid, is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. Methylmalonic acid is a key intermediary in the anaplerotic reactions of the Krebs cycle. These Dibasic acids can be separated, retained, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (FA). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid

HPLC Method for Methylmalonic Acid, Malonic Acid on Newcrom B by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Methylmalonic Acid, Malonic Acid .

Methylmalonic Acid is an organic acid with the chemical formula  C₄H₆O₄. It works as an intermediate in the breakdown of certain amino acids and fatty acids. Elevated levels of Methylmalonic avid can be a sign of a Vitamin B12 deficiency or Methylmalonic acidemia, which is a rare genetic disorder where the previously mentioned acid is not properly metabolized, leading to its accumulation.

Malonic Acid is an organic acid with the C₃H₄O₄ chemical formula.  It has a variety of uses from synthesis to preservatives. When it comes to synthesis, it is often used in industrial means. and especially dyes for natural fibers. Besides industrial use, in laboratory environments, it is used in preparation of tris-maleate, sodium maleate buffers, and maleate salts.

Methylmalonic Acid, Malonic Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using UV.

HPLC Method for Fumaric Acid, Methylmalonic Acid, Maleic Acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fumaric Acid, Methylmalonic Acid, Maleic Acid 

Methylmalonic Acid is an organic acid with the chemical formula  C₄H₆O₄. It works as an intermediate in the breakdown of certain amino acids and fatty acids. Elevated levels of Methylmalonic avid can be a sign of a Vitamin B12 deficiency or Methylmalonic acidemia, which is a rare genetic disorder where the previously mentioned acid is not properly metabolized, leading to its accumulation.

Malonic Acid is an organic acid with the C₃H₄O₄ chemical formula.  It has a variety of uses from synthesis to preservatives. When it comes to synthesis, it is often used in industrial means. and especially dyes for natural fibers. Besides industrial use, in laboratory environments, it is used in preparation of tris-maleate, sodium maleate buffers, and maleate salts.

Fumaric Acid, also known as trans-butenedioic acid, is an organic compound with C4H4O4 chemical formula. It is used across Food, industrial, and medical industries. In food, is it often used as a preservative, pH regulator, and flavoring akin to citric acid.  Industrially, it is used in making polyester resins, polyhydric alcohols, and more. Medically, it is used in denture cleaners and it’s derivatives are used in treating psoriasis.

Fumaric Acid, Methylmalonic Acid, Maleic Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.

HPLC Method for Glycolic acid, Malic Acid, Maleic Acid, Methylmalonic Acid, Succinic Acid, Tartaric Acid, dl-Tartaric acid, Fumaric Acid, Citric Acid, Malonic Acid, Gluconic acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Glycolic acid, Malic Acid, Maleic Acid, Methylmalonic Acid, Succinic Acid, Tartaric Acid, dl-Tartaric acid, Fumaric Acid, Citric Acid, Malonic Acid, Gluconic acid.

Gluconic Acid is an organic acid with the chemical formula C₆H₁₂O₇. It is naturally found in fruits. Industrially, it is found in honey that is produced by fermenting starch. Typically, it is used in food, beverage, cosmetic, and skin care industries.

Malic Acid is an alpha hydroxy acid with the chemical formula C₄H₆O₅. It is most commonly found in fruits and wines. It is sour, which is partially why it is often used as a food additive. Besides food, it is also used in skin-care as an exfoliant.

Citric Acid is a naturally occurring organic acid found in citrus fruits; it is also an intermediate in the citric acid cycle of aerobic organisms. It is used industrially as an acidity regulator, flavoring, detergent, and more than 2 million tons are produced annually. It’s chemical formula is C₆H₈O₇.

Tartaric Acid is an organic acid with the chemical formula C₄H₆O6. It is found in fruits like grapes and tamarinds and is a vital component of wine. It is also used in metal cleaning, as an antioxidant, and as an acidulant.

Malonic Acid is an organic compound with the chemical formula C₃H₄O₄. It is also known as cis-butenedioic acid. It is a trans counterpart of fumaric acid. It has a variety of uses from dyeing natural fibers to oil and fat preservative, to synthesis of hydrogels.

Fumaric Acid, also known as trans-butenedioic acid, is an organic compound with C4H4O4 chemical formula. It is used across Food, industrial, and medical industries. In food, is it often used as a preservative, pH regulator, and flavoring akin to citric acid. Industrially, it is used in making polyester resins, polyhydric alcohols, and more. Medically, it is used in denture cleaners and it’s derivatives are used in treating psoriasis.

Glycolic acid, Malic Acid, Maleic Acid, Methylmalonic Acid, Succinic Acid, Tartaric Acid, dl-Tartaric acid, Fumaric Acid, Citric Acid, Malonic Acid, Gluconic acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using CAD.

Organic and inorganic acids can be separated by reversed-phase anion-exchange Primesep D column. Succinic and methylmalonic acids were separated based on their hydrophobic and ionic properties. Method can be used for analysis of other acids by HPLC.

Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.

Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.